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首页> 外文期刊>Journal of Molecular Biology >Electrostatics in the ribosomal tunnel modulate chain elongation rates.
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Electrostatics in the ribosomal tunnel modulate chain elongation rates.

机译：核糖体通道中的静电调节链的延伸率。

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摘要

Electrostatic potentials along the ribosomal exit tunnel are nonuniform and negative. The significance of electrostatics in the tunnel remains relatively uninvestigated, yet they are likely to play a role in translation and secondary folding of nascent peptides. To probe the role of nascent peptide charges in ribosome function, we used a molecular tape measure that was engineered to contain different numbers of charged amino acids localized to known regions of the tunnel and measured chain elongation rates. Positively charged arginine or lysine sequences produce transient arrest (pausing) before the nascent peptide is fully elongated. The rate of conversion from transiently arrested to full-length nascent peptide is faster for peptides containing neutral or negatively charged residues than for those containing positively charged residues. We provide experimental evidence that extraribosomal mechanisms do not account for this charge-specific pausing. We conclude that pausing is due to charge-specific interactions between the tunnel and the nascent peptide.
机译：沿核糖体出口通道的静电势不均匀且为负。隧道中静电的重要性尚待研究，但它们可能在新生肽的翻译和二级折叠中发挥作用。为了探究新生肽电荷在核糖体功能中的作用，我们使用了一种分子卷尺，该分子卷尺经工程设计，可包含位于隧道已知区域的不同数量的带电氨基酸，并可以测量链的延伸率。带正电荷的精氨酸或赖氨酸序列在新生肽完全延长之前会产生短暂的停止（暂停）。含有中性或负电荷残基的肽比那些含有正电荷残基的肽从瞬时停滞肽到全长新生肽的转化速率更快。我们提供了实验证据，表明核糖体外机制不能解释这种电荷特异性暂停。我们得出结论，暂停是由于隧道与新生肽之间的电荷特异性相互作用。

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