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首页> 外文期刊>Journal of Molecular Biology >Ribosome-small-subunit-dependent GTPase interacts with tRNA-binding sites on the ribosome.
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Ribosome-small-subunit-dependent GTPase interacts with tRNA-binding sites on the ribosome.

机译：核糖体小亚基依赖性GTP酶与核糖体上的tRNA结合位点相互作用。

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摘要

RsgA (ribosome-small-subunit-dependent GTPase A, also known as YjeQ) is a unique GTPase in that guanosine triphosphate hydrolytic activity is activated by the small subunit of the ribosome. Disruption of the gene for RsgA from the genome affects the growth of cells, the subunit association of the ribosome, and the maturation of 16S rRNA. To study the interaction of Escherichia coli RsgA with the ribosome, chemical modifications using dimethylsulfate and kethoxal were performed on the small subunit in the presence or in the absence of RsgA. The chemical reactivities at G530, A790, G925, G926, G966, C1054, G1339, G1405, A1413, and A1493 in 16S rRNA were reduced, while those at A532, A923, G1392, A1408, A1468, and A1483 were enhanced, by the addition of RsgA, together with 5'-guanylylimidodiphosphate. Among them, the chemical reactivities at A532, A790, A923, G925, G926, C1054, G1392, A1413, A1468, A1483, and A1493 were not changed when RsgA was added together with GDP. These results indicate that the binding of RsgA induces conformational changes around the A site, P site, and helix 44, and that guanosine triphosphate hydrolysis induces partial conformational restoration, especially in the head, to dissociate RsgA from the small subunit. RsgA has the capacity to coexist with mRNA in the ribosome while it promotes dissociation of tRNA from the ribosome.
机译：RsgA（依赖核糖体-小亚基的GTPase A，也称为YjeQ）是一种独特的GTPase，因为三磷酸鸟苷的水解活性被核糖体的小亚基激活。 RsgA基因从基因组中的破坏影响细胞的生长，核糖体的亚基缔合以及16S rRNA的成熟。为了研究大肠杆菌RsgA与核糖体的相互作用，在有或没有RsgA的情况下，对小亚基进行了使用硫酸二甲酯和乙二醛的化学修饰。降低了16S rRNA中G530，A790，G925，G926，G926，G966，C1054，G1339，G1405，A1413和A1493的化学反应性，同时增强了A532，A923，G1392，A1408，A1468和A1483的化学反应性。加入RsgA，以及5'-鸟苷基二磷酸。其中，当将RsgA与GDP一起添加时，A532，A790，A923，G925，G926，C1054，G1392，A1413，A1468，A1483和A1493的化学反应性不变。这些结果表明RsgA的结合诱导A位，P位和螺旋44周围的构象变化，鸟苷三磷酸水解诱导部分构象恢复，尤其是在头部，从而使RsgA与小亚基解离。 RsgA具有与核糖体中的mRNA共存的能力，同时它促进tRNA从核糖体中解离。

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