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首页> 外文期刊>Journal of Molecular Biology >Assembly of the 5 ' and 3 ' minor domains of 16S ribosomal RNA as monitored by tethered probing from ribosomal protein S20
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Assembly of the 5 ' and 3 ' minor domains of 16S ribosomal RNA as monitored by tethered probing from ribosomal protein S20

机译：16S核糖体RNA的5'和3'小结构域的组装，通过从核糖体蛋白S20的系链探测进行监测

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The ribosomal protein (r-protein) S20 is a primary binding protein. As such, it interacts directly and independently with the 5' domain as well as the 3' minor domain of 16S ribosomal RNA (rRNA) in minimal particles and the fully assembled 30S subunit. The interactions observed between r-protein S20 and the 5' domain of 16S rRNA are quite extensive, while those between r-protein S20 and the 3' minor domain are significantly more limited. In this study, directed hydroxyl radical probing mediated by Fe(II)-derivatized S20 proteins was used to monitor the folding of 16S rRNA during r-protein association and 30S subunit assembly. An analysis of the cleavage patterns in the minimal complexes [16S rRNA and Fe(II)-S20] and the fully assembled 30S subunit containing the same Fe(II)-derivatized proteins shows intriguing similarities and differences. These results suggest that the two domains, 5' and 3' minor, are organized relative to S20 at different stages of assembly. The 5' domain acquires, in a less complex ribonucleoprotein particle than the 3' minor domain, the same architecture as observed in mature subunits. These results are similar to what would be predicted of subunit assembly by the 5'-to-3' direction assembly model. (C) 2007 Elsevier Ltd. All rights reserved.
机译：核糖体蛋白（r蛋白）S20是主要的结合蛋白。因此，它与16S核糖体RNA（rRNA）的5'结构域和3'次要结构域直接且独立地相互作用，并具有最小的颗粒和完全组装的30S亚基。 r蛋白S20与16S rRNA 5'结构域之间的相互作用非常广泛，而r蛋白S20与3'次要结构域之间的相互作用则受到更大的限制。在这项研究中，由Fe（II）衍生的S20蛋白介导的定向羟基自由基探测用于监测r蛋白缔合和30S亚基组装过程中16S rRNA的折叠。最小复合物[16S rRNA和Fe（II）-S20]和包含相同Fe（II）衍生蛋白的完全组装的30S亚基的切割模式分析显示出令人着迷的相似性和差异。这些结果表明，在组装的不同阶段，相对于S20，两个结构域（5'和3'小）被组织。 5'结构域在比3'次要结构域更复杂的核糖核蛋白颗粒中获得与成熟亚基相同的结构。这些结果类似于通过5'到3'方向组装模型预测的亚基组装。 （C）2007 Elsevier Ltd.保留所有权利。

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