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首页> 外文期刊>Journal of Molecular Biology >Three-dimensional structures of Pseudomonas aeruginosa PvcA and PvcB, two proteins involved in the synthesis of 2-isocyano-6,7-dihydroxycoumarin.
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Three-dimensional structures of Pseudomonas aeruginosa PvcA and PvcB, two proteins involved in the synthesis of 2-isocyano-6,7-dihydroxycoumarin.

机译：铜绿假单胞菌PvcA和PvcB的三维结构，这两种蛋白参与了2-isocyano-6,7-dihydroxycoumarin的合成。

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摘要

The pvcABCD operon of Pseudomonas aeruginosa encodes four proteins (PA2254, PA2255, PA2256, and PA2257) that form a cluster that is responsible for the synthesis of a cyclized isocyano derivative of tyrosine. These proteins, which were identified originally as being responsible for a step in the maturation of the chromophore of the peptide siderophore pyoverdine, have been identified recently as belonging to a family of proteins that produce small organic isonitriles. We report that strains harboring a disruption in the pvcA or pvcB genes are able to grow in iron-depleted conditions and to produce pyoverdine. Additionally, we have determined the three-dimensional crystal structures of PvcA and PvcB. The structure of PvcA demonstrates a novel enzyme architecture that is built upon a Rossmann fold. We have analyzed the sequence conservation of enzymes within this family and identified six conserved motifs. These regions of the protein cluster around a putative active site cavity. The structure of the PvcB protein confirms it is a member of the Fe2+/alpha-ketoglutarate-dependent oxygenase family of enzymes. The active site of PvcB is compared to the structures of other family members and suggests that a conformational change to order several loops will accompany the binding of ligands.
机译：铜绿假单胞菌的pvcABCD操纵子编码四种蛋白质（PA2254，PA2255，PA2256和PA2257），这些蛋白质形成一个簇，负责合成酪氨酸的环化异氰基衍生物。这些蛋白质最初被鉴定为负责肽铁载体吡啶酮的发色团成熟的步骤，最近已被鉴定为属于产生小型有机异腈的蛋白质家族。我们报告说，在pvcA或pvcB基因中含有破坏的菌株能够在贫铁条件下生长并产生pyoverdine。此外，我们确定了PvcA和PvcB的三维晶体结构。 PvcA的结构展示了建立在Rossmann折叠上的新型酶结构。我们已经分析了该家族中酶的序列保守性，并鉴定了六个保守基序。蛋白质的这些区域聚集在假定的活性位点腔周围。 PvcB蛋白的结构证实它是Fe2 + /α-酮戊二酸依赖性加氧酶家族的成员。 PvcB的活性位点与其他家族成员的结构进行了比较，并表明构象变化以排序几个环将伴随配体的结合。

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