A deoxyribozyme with a novel guanine quartet-helix pseudoknot structure

McManus SA; Li YF

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A deoxyribozyme with a novel guanine quartet-helix pseudoknot structure

机译：具有新型鸟嘌呤四螺旋-螺旋假结结构的脱氧核酶

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Here we report a deoxyribozyme with a unique structure that contains a two-tiered guanine quadruplex interlinked to a Watson-Crick duplex. Through in vitro selection, sequence mutation, and methylation interference, we show the presence of both the two-tiered guanine-quadruplex and two helical regions contained in the active structure of this self-phosphorylating deoxyribozyme. Interestingly, one GG element of the quadruplex is part of a hairpin loop within one of the identified helical regions. Circular dichroism analysis showed that antiparallel quadruplex formation was dependent on this helix. To our knowledge, this is the first report of a pseudoknot nucleic acid structure that involves a guanine quadruplex. Our findings indicate that guanine quadruplexes can be part of complex structural arrangements, increasing the likelihood of finding more complex guanine quadruplex arrangements in biological systems. (c) 2007 Elsevier Ltd. All rights reserved.

机译：在这里，我们报告了一种具有独特结构的脱氧核酶，该结构包含一个与Watson-Crick双链体互连的两层鸟嘌呤四链体。通过体外选择，序列突变和甲基化干扰，我们表明在这种自磷酸化脱氧核酶的活性结构中包含两个鸟嘌呤-四链体和两个螺旋区域的存在。有趣的是，四链体的一个GG元素是所识别的螺旋区域之一内的发夹环的一部分。圆二色性分析表明，反平行四链体的形成取决于该螺旋。据我们所知，这是涉及鸟嘌呤四链体的假结核酸结构的首次报道。我们的研究结果表明鸟嘌呤四联体可能是复杂的结构安排的一部分，增加了在生物系统中发现更复杂的鸟嘌呤四联体安排的可能性。（c）2007 Elsevier Ltd.保留所有权利。

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《Journal of Molecular Biology》 |2008年第4期|共9页
作者
McManus SA; Li YF;
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正文语种 eng
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deoxyribozyme; DNA phosphorylation; guanine quadruplex; pseudoknot; in vitro selection; G-QUADRUPLEX; PROMOTER REGION; TELOMERIC DNA; CLEAVING DNA; ACID; ENZYMES; MOTIFS;

机译：脱氧核酶;DNA磷酸化;鸟嘌呤四联体;假单胞胎;体外选择;G-四倍体;启动子区域;端粒DNA;裂解DNA;酸;酶;MOTIFS;

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专利

1. A deoxyribozyme with a novel guanine quartet-helix pseudoknot structure [J] . McManus SA, Li YF Journal of Molecular Biology . 2008,第4期

机译：具有新型鸟嘌呤四螺旋-螺旋假结结构的脱氧核酶
2. Chlamydomonas reinhardtii telomere repeats form unstable structures involving guanine-guanine base pairs [J] . Judith Berman, Marie E. Petracek Nucleic acids research . 1992,第1期

机译：莱茵衣藻端粒重复序列形成不稳定结构，涉及鸟嘌呤-鸟嘌呤碱基对
3. Chlamydomonas reinhardtii telomere repeats form unstable structures involving guanine-guanine base pairs [J] . Judith Berman, Marie E. Petracek Nucleic acids research . 1992,第1期

机译：莱茵衣藻端粒重复序列形成不稳定结构，涉及鸟嘌呤-鸟嘌呤碱基对
4. The Effects of Split Valence Basis Sets on Muon Hyperfine Interaction in Guanine Nucleobase and Nucleotide Structures [C] . Wan Nurfadhilah Zaharim, Shukri Sulaiman, Siti Nuramira Abu Bakar, International Conference on Functional Materials Science . 2019

机译：分裂价基集对鸟嘌呤核碱基和核苷酸结构中的μm杂种相互作用的影响
5. I. Comparison of translesion bypass of guanine-N2 monoadducts of mitomycin C and guanine -N7 monoadducts of 2,7-diaminomitosene by T7 exo-, Klenow exo-, eta and Klenow exo+ DNA polymerases. II. Structure-based design, synthesis, structure-conformation and structure -activity relationships studies of D-Phe-Pro-D-Arg-P1'-CONH2 tetrapeptides with inhibitory activity for thrombin. [D] . Clement, Cristina C. 2006

机译：I.通过T7 exo-，Klenow exo-，eta和Klenow exo + DNA聚合酶比较丝裂霉素C的鸟嘌呤-N2单加合物和2,7-二氨基光油的鸟嘌呤-N7单加合物的跨病变旁路。二。具有凝血酶抑制活性的D-Phe-Pro-D-Arg-P1'-CONH2四肽的基于结构的设计，合成，结构构象和结构-活性关系研究。
6. Chlamydomonas reinhardtii telomere repeats form unstable structures involving guanine-guanine base pairs. [O] . M E Petracek, J Berman 1992

机译：莱茵衣藻端粒重复序列形成不稳定结构涉及鸟嘌呤-鸟嘌呤碱基对。
7. Chlamydomonas reinhardtii telomere repeats form unstable structures involving guanine-guanine base pairs. [O] . Petracek, M E, Berman, J 1992

机译：莱茵衣藻端粒重复序列形成涉及鸟嘌呤-鸟嘌呤碱基对的不稳定结构。

A deoxyribozyme with a novel guanine quartet-helix pseudoknot structure

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