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首页> 外文期刊>Journal of Molecular Biology >Direct optical microscopic observation of the microtubule polymerization intermediate sheet structure in the presence of gas7.
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Direct optical microscopic observation of the microtubule polymerization intermediate sheet structure in the presence of gas7.

机译：在气体存在下对微管聚合中间片结构的直接光学显微镜观察。

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摘要

The process of microtubule elongation is thought to consist of two stages-formation of a tubulin sheet structure and its closure into a tube. However, real-time observation of this process has been difficult. Here, by utilizing phospho-tau binding protein Gas7 (growth-arrest-specific protein 7), we visualized the polymer transformation process by dark-field microscopy. Upon elongation, thin and flexible structures, often similar to a curved hook, appeared at the end of microtubules. Electron microscopic observations supported the idea that these flexible structures are tubulin sheets. They maintained their length until they gradually became thick and rigid beginning in the central portion, resulting in straight microtubules. In the absence of Gas7, the sheet-like structure was rarely observed; moreover, when observed, it was fragile and engaged in typical dynamic instability. With Gas7, no catastrophe was observed. These results suggest that Gas7 enhances microtubule polymerization by stabilizing sheet intermediates and is a useful tool for analyzing microtubule transformation.
机译：微管延长的过程被认为包括两个阶段：微管蛋白片结构的形成和其封闭成管的过程。但是，很难实时观察该过程。在这里，通过利用磷酸化-tau结合蛋白Gas7（生长抑制特异性蛋白7），我们通过暗场显微镜观察了聚合物的转化过程。伸长后，微管末端出现薄而柔软的结构，通常类似于弯曲的钩。电子显微镜观察支持了这些柔性结构是微管蛋白片的想法。它们保持其长度，直到从中央部分开始逐渐变厚并变硬，从而形成直的微管。在没有Gas7的情况下，很少观察到片状结构。而且，当观察时，它是脆弱的并且处于典型的动态不稳定中。使用Gas7，未发现任何灾难。这些结果表明，Gas7通过稳定片状中间体来增强微管聚合，并且是分析微管转化的有用工具。

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