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首页> 外文期刊>Journal of Molecular Biology >Structure and specificity of a binary tandem domain F-lectin from striped bass (Morone saxatilis).
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Structure and specificity of a binary tandem domain F-lectin from striped bass (Morone saxatilis).

机译：条纹鲈（Morone saxatilis）的二进制串联结构域F-凝集素的结构和特异性。

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摘要

The plasma of the striped bass Morone saxatilis contains a fucose-specific lectin (MsaFBP32) that consists of two F-type carbohydrate recognition domains (CRDs) in tandem. The crystal structure of the complex of MsaFBP32 with l-fucose reported here shows a cylindrical 81-A-long and 60-A-wide trimer divided into two globular halves: one containing N-terminal CRDs (N-CRDs) and the other containing C-terminal CRDs (C-CRDs). The resulting binding surfaces at the opposite ends of the cylindrical trimer have the potential to cross-link cell surface or humoral carbohydrate ligands. The N-CRDs and C-CRDs of MsaFBP32 exhibit significant structural differences, suggesting that they recognize different glycans. Analysis of the carbohydrate binding sites provides the structural basis for the observed specificity of MsaFBP32 for simple carbohydrates and suggests that the N-CRD recognizes more complex fucosylated oligosaccharides and with a relatively higher avidity than the C-CRD. Modeling of MsaFBP32 complexed with fucosylated glycans that are widely distributed in prokaryotes and eukaryotes rationalizes the observation that binary tandem CRD F-type lectins function as opsonins by cross-linking "non-self" carbohydrate ligands and "self" carbohydrate ligands, such as sugar structures displayed by microbial pathogens and glycans on the surface of phagocytic cells from the host.
机译：条纹鲈鱼Morone saxatilis的血浆包含岩藻糖特异性凝集素（MsaFBP32），由串联的两个F型碳水化合物识别域（CRD）组成。 MsaFBP32与L-岩藻糖的复合物的晶体结构显示，圆柱状的81-A长和60-A宽的三聚体分为两个球形部分：一个包含N末端CRD（N-CRD），另一个包含C端CRD（C-CRD）。在圆柱形三聚体的相对端处产生的结合表面具有使细胞表面或体液碳水化合物配体交联的潜力。 MsaFBP32的N-CRD和C-CRD表现出明显的结构差异，表明它们识别不同的聚糖。碳水化合物结合位点的分析为观察到的MsaFBP32对简单碳水化合物的特异性提供了结构基础，并建议N-CRD识别比C-CRD更复杂的岩藻糖基化寡糖，并且具有相对更高的亲和力。与广泛存在于原核生物和真核生物中的岩藻糖基化聚糖复合的MsaFBP32的建模合理化了以下观察结果：二元串联CRD F型凝集素通过交联“非自身”碳水化合物配体和“自身”碳水化合物配体（如糖）而起调理素的作用。由宿主的吞噬细胞表面上的微生物病原体和聚糖显示的结构。

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