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首页> 外文期刊>Journal of Molecular Biology >Structure and function of P19, a high-affinity iron transporter of the human pathogen Campylobacter jejuni.
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Structure and function of P19, a high-affinity iron transporter of the human pathogen Campylobacter jejuni.

机译：人类病原体空肠弯曲菌的高亲和力铁转运体P19的结构和功能。

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摘要

Campylobacter jejuni, a major cause of acute bacterial diarrhea in humans, expresses numerous proteins to import diverse forms of essential iron. The expression of p19 and an adjacent iron transporter homologue (ftr1) is strongly induced upon iron limitation, suggesting a function in iron acquisition. Here, we show that the loss of P19 alone is detrimental to growth on iron-restricted media. Furthermore, metal binding analysis demonstrates that recombinant P19 has distinct copper and iron binding sites. Crystal structures of P19 have been solved to 1.41 A resolution, revealing an immunoglobulin-like fold. A P19 homodimer in which both monomers contribute ligands to two equivalent copper sites located adjacent to methionine-rich patches is observed. Copper coordination occurs via three histidine residues (His42, His95, and His132) and Met88. A solvent channel lined with conserved acidic residues leads to the copper site. Soaking crystals with a solution of manganese as iron analog reveals a second metal binding site in this solvent channel (metal-metal distance, 7.7 A). Glu44 lies between the metal sites and displays multiple conformations in the crystal structures, suggesting a role in regulating metal-metal interaction. Dimerization is shown to be metal dependent in vitro and is detected in vivo by cross-linking.
机译：空肠弯曲菌是人类急性细菌性腹泻的主要原因，它表达多种蛋白质以输入各种形式的必需铁。 p19和相邻的铁转运蛋白同源物（ftr1）的表达在铁限制条件下被强烈诱导，提示其在铁摄取中的功能。在这里，我们表明仅P19的丢失对铁限制培养基上的生长是有害的。此外，金属结合分析表明重组P19具有独特的铜和铁结合位点。 P19的晶体结构已解析到1.41 A的分辨率，显示出类似免疫球蛋白的折叠。观察到一种P19同型二聚体，其中两种单体均向位于与富甲硫氨酸斑块相邻的两个等效铜位点贡献配体。铜配位通过三个组氨酸残基（His42，His95和His132）和Met88发生。内衬有保守酸性残基的溶剂通道通向铜位。用锰溶液作为铁类似物浸泡晶体后，在该溶剂通道中发现了第二个金属结合位点（金属与金属的距离为7.7 A）。 Glu44位于金属位点之间，并在晶体结构中显示出多种构象，表明在调节金属与金属的相互作用中起作用。已显示二聚化在体外是金属依赖性的，并且在体内通过交联检测。

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