• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Molecular Biology >Local rather than global folding enables the lead-dependent activity of the 8-17 deoxyribozyme: evidence from contact photo-crosslinking.
【24h】

Local rather than global folding enables the lead-dependent activity of the 8-17 deoxyribozyme: evidence from contact photo-crosslinking.

机译：局部折叠而不是整体折叠使8-17脱氧核酶的铅依赖性活性成为可能：接触光交联的证据。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The 8-17 deoxyribozyme is an in vitro selected enzyme capable of sequence-specific cleavage of RNA. While selected to be a magnesium and zinc-utilizing enzyme, the 8-17 DNAzyme has been shown to utilize lead for its catalysis. Fluorescence-based experiments have indicated that the magnesium- and zinc-utilizing versions of the DNAzyme-substrate complex need to form a defined tertiary structure to be active, but no such global folding is required for the lead-mediated activity. Here, we have investigated this phenomenon, including the use of contact photo-crosslinking to map the tertiary fold of the lead-dependent DNAzyme. While our results recapitulate that global folding is not required for the lead activity, they reveal strikingly distinct lead-mediated modes of activity under conditions of low versus moderate solution ionic strength. Even in very low salt buffers, where no global folding of the 8-17 DNAzyme occurs, the active site of the enzyme appears to form a distinct local fold, one that cannot be modelled easily by DNA/RNA constructs that preserve key sequence and secondary structure features of the active site.
机译：8-17脱氧核酶是一种体外选择的能够对RNA进行序列特异性切割的酶。虽然被选为利用镁和锌的酶，但已证明8-17 DNAzyme可利用铅进行催化。基于荧光的实验表明，DNA酶-底物复合物的利用镁和锌的形式需要形成确定的三级结构才能具有活性，但是对于铅介导的活性则不需要这种全局折叠。在这里，我们已经研究了这种现象，包括使用接触光交联来绘制铅依赖性DNAzyme的三级折叠图。虽然我们的结果概括了铅活性不需要全局折叠，但它们揭示了在溶液离子强度较低与中等的条件下，铅介导的活性模式截然不同。即使在盐浓度很低的缓冲液中，也不会发生8-17 DNAzyme的整体折叠，该酶的活性位点似乎会形成一个独特的局部折叠，而保留关键序列和次级序列的DNA / RNA构建体则无法轻易地模拟这种折叠。活动站点的结构特征。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号