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首页> 外文期刊>Journal of Molecular Biology >A bacterial GAP-like protein, YihI, regulating the GTPase of Der, an essential GTP-binding protein in Escherichia coli.
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A bacterial GAP-like protein, YihI, regulating the GTPase of Der, an essential GTP-binding protein in Escherichia coli.

机译：细菌GAP样蛋白YihI调节Der的GTP酶，Der是大肠杆菌中一种必需的GTP结合蛋白。

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摘要

Der (double Era-like GTPase) is an essential GTPase consisting of two GTP-binding motifs in tandem followed by a KH-like domain. Der plays a critical role in 50S ribosome maturation at a later biogenesis step. Here, we attempted to identify a protein interacting with Der that modulates its function and regulation. Using a yeast two-hybrid, we discovered that Der interacts with YihI, which activates the GTPase activity of Der. Its overexpression affected cell growth, causing accumulation of rRNA precursors and an aberrant ribosome profile that was similar to that of Der-depleted cells, suggesting that Der and YihI are involved in the 50S ribosome assembly. The yihI deletion strain showed a shorter lag phase than wild-type strain, suggesting that YihI may be a negative regulator for ribosome assembly. We propose that YihI is a GAP (GTPase-activating protein)-like protein that modulates Der function to negatively regulate cell growth at the beginning of exponential growth.
机译：Der（双重Era样GTP酶）是必不可少的GTP酶，由两个GTP结合基序串联在一起，后跟一个KH样结构域。在随后的生物发生步骤中，Der在50S核糖体成熟中起关键作用。在这里，我们试图鉴定与Der相互作用的蛋白，该蛋白可调节其功能和调控。使用酵母双杂交，我们发现Der与YihI相互作用，从而激活Der的GTPase活性。它的过表达影响细胞的生长，导致rRNA前体的积累和异常的核糖体概况，与耗尽Der的细胞相似，表明Der和YihI参与了50S核糖体装配。 yihI缺失菌株显示出比野生型菌株更短的滞后阶段，表明YihI可能是核糖体装配的负调控因子。我们提出YihI是一种GAP（GTPase激活蛋白）样蛋白，可调节Der功能以在指数增长开始时负调控细胞生长。

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