• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Molecular Biology >The branched photocycle of the slow-cycling channelrhodopsin-2 mutant C128T.
【24h】

The branched photocycle of the slow-cycling channelrhodopsin-2 mutant C128T.

机译：慢循环channelrhodopsin-2突变体C128T的分支光周期。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Channelrhodopsins (ChRs) of green algae such as Chlamydomonas are used as neuroscience tools to specifically depolarize cells with light. A crude model of the ChR2 photocycle has been recently established, but details of the photoreactions are widely unknown. Here, we present the photoreactions of a slow-cycling ChR2 mutant (step function rhodopsin), with C128 replaced by threonine and 200-fold extended lifetime of the conducting-state P520. At a late state of the photocycle, a fraction of the proteins branches off into an inactive species, P380, which accumulates during prolonged illumination. At neutral pH, P380 is converted into P353, a species with a characteristic fine-structured spectrum that is interpreted as retroretinyl chromophore. The described branching reactions should be considered, when ChR is used as a neuroscience tool, especially in the case of fluorescence imaging at high light intensities.
机译：绿藻（如衣藻）的通道视紫红质（ChRs）被用作神经科学工具，以使光特异性地使细胞去极化。最近已经建立了ChR2光循环的粗略模型，但是光反应的细节却广为人知。在这里，我们介绍了一个慢循环ChR2突变体（阶梯功能视紫红质）的光反应，其中C128被苏氨酸取代，导电状态P520的寿命延长了200倍。在光周期的后期，一部分蛋白质分支成无活性物质，即P380，该物质在长时间的照明过程中会积聚。在中性pH值下，P380转化为P353，这是一种具有特征化的精细结构光谱的物质，被解释为逆视黄基发色团。当ChR用作神经科学工具时，尤其是在高光强度下进行荧光成像的情况下，应考虑所描述的支化反应。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号