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首页> 外文期刊>Journal of Molecular Biology >Mutations targeting the C-terminal domain of FliG can disrupt motor assembly in the Na(+)-driven flagella of Vibrio alginolyticus.
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Mutations targeting the C-terminal domain of FliG can disrupt motor assembly in the Na(+)-driven flagella of Vibrio alginolyticus.

机译：针对FliG的C末端域的突变可以破坏藻解弧菌的Na（+）驱动鞭毛中的马达组装。

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The torque of the bacterial flagellar motor is generated by the rotor-stator interaction coupled with specific ion translocation through the stator channel. To produce a fully functional motor, multiple stator units must be properly incorporated around the rotor by an as yet unknown mechanism to engage the rotor-stator interactions. Here, we investigated stator assembly using a mutational approach of the Na(+)-driven polar flagellar motor of Vibrio alginolyticus, whose stator is localized at the flagellated cell pole. We mutated a rotor protein, FliG, which is located at the C ring of the basal body and closely participates in torque generation, and found that point mutation L259Q, L270R or L271P completely abolishes both motility and polar localization of the stator without affecting flagellation. Likewise, mutations V274E and L279P severely affected motility and stator assembly. Those residues are localized at the core of the globular C-terminal domain of FliG when mapped onto the crystal structure of FliG from Thermotoga maritima, which suggests that those mutations induce quite large structural alterations at the interface responsible for the rotor-stator interaction. These results show that the C-terminal domain of FliG is critical for the proper assembly of PomA/PomB stator complexes around the rotor and probably functions as the target of the stator at the rotor side.
机译：细菌鞭毛马达的转矩是由转子-定子相互作用以及通过定子通道的特定离子易位产生的。为了生产全功能的电动机，必须通过一种尚不为人所知的机构将多个定子单元正确地结合在转子周围，以使转子与定子相互作用。在这里，我们研究了突变体的方法，该方法采用的是Na（+）驱动的溶藻弧菌极性鞭毛马达的定子突变体，其定子位于鞭毛细胞极。我们突变了位于基体C环并紧密参与扭矩产生的转子蛋白FliG，发现点突变L259Q，L270R或L271P完全消除了定子的运动性和极性定位，而没有影响鞭毛。同样，突变V274E和L279P严重影响了运动性和定子装配。当将这些残基定位到来自海栖嗜热菌的FliG的晶体结构上时，它们位于FliG球状C末端结构域的核心，这表明这些突变在负责转子-定子相互作用的界面上诱导了相当大的结构改变。这些结果表明，FliG的C端域对于在转子周围正确组装PomA / PomB定子复合体至关重要，并且可能在转子侧充当定子的目标。

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