• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Molecular Biology >RNA polymerase II and TAFs undergo a slow isomerization after the polymerase is recruited to promoter-bound TFIID.
【24h】

RNA polymerase II and TAFs undergo a slow isomerization after the polymerase is recruited to promoter-bound TFIID.

机译：RNA聚合酶II和TAF在募集到启动子结合的TFIID之后，会经历缓慢的异构化。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Transcription of mRNA genes requires that RNA polymerase II (Pol II) and the general transcription factors assemble on promoter DNA to form an organized complex capable of initiating transcription. Biochemical studies have shown that Pol II and TFIID (transcription factor IID) contact overlapping regions of the promoter, leading to the question of how these large factors reconcile their promoter interactions during complex assembly. To investigate how the TAF (TATA-binding protein-associated factor) subunits of TFIID alter the kinetic mechanism by which complexes assemble on promoters, we used a highly purified human transcription system. We found that TAFs sharply decrease the rate at which Pol II, TFIIB, and TFIIF assemble on promoter-bound TFIID-TFIIA. Interestingly, the slow step in this process is not recruitment of these factors to the DNA, but rather a postrecruitment isomerization of protein-DNA contacts that occurs throughout the core promoter. Our findings support a model in which Pol II and the general transcription factors rapidly bind promoter-bound TFIID-TFIIA, after which complexes undergo a slow isomerization in which the TAFs reorganize their contacts with the promoter to allow Pol II to properly engage the DNA. In this manner, TAFs kinetically repress basal transcription.
机译：mRNA基因的转录需要RNA聚合酶II（Pol II）和一般转录因子在启动子DNA上组装，以形成能够启动转录的有组织的复合体。生化研究表明，Pol II和TFIID（转录因子IID）接触启动子的重叠区域，从而引发了一个问题，即这些大因子如何在复杂装配过程中协调其启动子相互作用。为了研究TFIID的TAF（TATA结合蛋白相关因子）亚基如何改变复合物在启动子上组装的动力学机制，我们使用了高度纯化的人类转录系统。我们发现，TAFs急剧降低了Pol II，TFIIB和TFIIF在启动子结合的TFIID-TFIIA上组装的速率。有趣的是，该过程中的缓慢步骤不是将这些因素募集到DNA中，而是整个核心启动子中发生的蛋白质-DNA接触的招聘后异构化。我们的发现支持了其中Pol II和一般转录因子快速结合启动子结合的TFIID-TFIIA的模型，此后复合物经历了缓慢的异构化，其中TAF重组了它们与启动子的接触，从而使Pol II能够正确地与DNA结合。以这种方式，TAF在动力学上抑制基础转录。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号