• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Molecular Biology >RNase III participates in GadY-dependent cleavage of the gadX-gadW mRNA.
【24h】

RNase III participates in GadY-dependent cleavage of the gadX-gadW mRNA.

机译：RNA酶III参与gadX-gadW mRNA的GadY依赖性切割。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The adjacent gadX and gadW genes encode transcription regulators that are part of a complex regulatory circuit controlling the Escherichia coli response to acid stress. We previously showed that the small RNA GadY positively regulates gadX mRNA levels. The gadY gene is located directly downstream of the gadX coding sequence on the opposite strand of the chromosome. We now report that gadX is transcribed in an operon with gadW, although this full-length mRNA does not accumulate. Base pairing of the GadY small RNA with the intergenic region of the gadX-gadW mRNA results in directed processing events within the region of complementarity. The resulting two halves of the cleaved mRNA accumulate to much higher levels than the unprocessed mRNA. We examined the ribonucleases required for this processing, and found that multiple enzymes are involved in the GadY-directed cleavage including the double-strand RNA-specific endoribonuclease RNase III.
机译：相邻的gadX和gadW基因编码转录调节子，该转录调节子是控制大肠杆菌对酸胁迫响应的复杂调节电路的一部分。我们先前显示，小RNA GadY正调控gadX mRNA水平。 gadY基因位于染色体相反链上gadX编码序列的直接下游。现在，我们报告gadX在带有gadW的操纵子中转录，尽管这种全长mRNA不会积累。 GadY小RNA与gadX-gadW mRNA的基因间区域的碱基配对导致互补区域内的定向加工事件。所得的两半切割的mRNA积累的水平比未加工的mRNA高得多。我们检查了此过程所需的核糖核酸酶，发现多种酶参与了GadY定向的切割，包括双链RNA特异性内切核糖核酸酶RNase III。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号