Crystal structure of the phage T4 recombinase UvsX and its functional interaction with the T4 SF2 helicase UvsW.

Gajewski S; Webb MR; Galkin V; Egelman EH; Kreuzer KN; White SW

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Crystal structure of the phage T4 recombinase UvsX and its functional interaction with the T4 SF2 helicase UvsW.

机译：噬菌体T4重组酶UvsX的晶体结构及其与T4 SF2解旋酶UvsW的功能相互作用。

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Bacteriophage T4 provides an important model system for studying the mechanism of homologous recombination. We have determined the crystal structure of the T4 UvsX recombinase, and the overall architecture and fold closely resemble those of RecA, including a highly conserved ATP binding site. Based on this new structure, we reanalyzed electron microscopy reconstructions of UvsX-DNA filaments and docked the UvsX crystal structure into two different filament forms: a compressed filament generated in the presence of ADP and an elongated filament generated in the presence of ATP and aluminum fluoride. In these reconstructions, the ATP binding site sits at the protomer interface, as in the RecA filament crystal structure. However, the environment of the ATP binding site is altered in the two filament reconstructions, suggesting that nucleotide cannot be as easily accommodated at the protomer interface of the compressed filament. Finally, we show that the phage helicase UvsW completes the UvsX-promoted strand-exchange reaction, allowing the generation of a simple nicked circular product rather than complex networks of partially exchanged substrates.

机译：噬菌体T4为研究同源重组的机制提供了重要的模型系统。我们已经确定了T4 UvsX重组酶的晶体结构，其总体结构和折叠与RecA的相似，包括一个高度保守的ATP结合位点。基于这种新结构，我们重新分析了UvsX-DNA细丝的电子显微镜重构，并将UvsX晶体结构对接成两种不同的细丝形式：在ADP存在下产生的压缩细丝和在ATP和氟化铝存在下产生的细丝。在这些重建过程中，ATP结合位点与RecA细丝晶体结构一样位于前体界面。但是，在两个细丝重构中，ATP结合位点的环境发生了变化，这表明核苷酸不能很容易地容纳在压缩细丝的前体界面上。最后，我们表明噬菌体解旋酶UvsW完成了UvsX促进的链交换反应，从而允许生成简单的带切口的圆形产物，而不是部分交换的底物的复杂网络。

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《Journal of Molecular Biology》 |2011年第1期|共12页
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Gajewski S; Webb MR; Galkin V; Egelman EH; Kreuzer KN; White SW;
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1. Crystal structure of the phage T4 recombinase UvsX and its functional interaction with the T4 SF2 helicase UvsW. [J] . Gajewski S, Webb MR, Galkin V, Journal of Molecular Biology . 2011,第1期

机译：噬菌体T4重组酶UvsX的晶体结构及其与T4 SF2解旋酶UvsW的功能相互作用。
2. Double-strand break repair and recombination-dependent replication of DNA in bacteriophage T4 in the absence of UvsX recombinase: Replicative resolution pathway [J] . ShcherbakovV.P., PluginaL., ShcherbakovaT., DNA repair . 2012,第5期

机译：在不存在UvsX重组酶的情况下，噬菌体T4中DNA的双链断裂修复和重组依赖复制：复制解决途径
3. Double-strand break repair and recombination-dependent replication of DNA in bacteriophage T4 in the absence of UvsX recombinase: Replicative resolution pathway [J] . ShcherbakovV.P., PluginaL., ShcherbakovaT., DNA repair . 2012,第5期

机译：在没有UVSX重组酶的情况下，在噬菌体T4中DNA的双链断裂修复和重组依赖性复制：复制分辨率途径
4. Inactivation of E. Coli, B. Subtilis Spores, and MS2, T4, and T7 Phage Using UV/H2O2 Advanced Oxidation [C] . Hadas Mamane, Hilla Shemer, Karl G. Linden World Congress on Ozone and Ultraviolet Technologies . 2007

机译：使用UV / H 2 O 2高级氧化灭活大肠杆菌，B.枯草芽孢杆菌孢子和MS2，T4和T7噬菌体
5. Analysis of the interactions between the 5' to 3' exonuclease and the single-stranded DNA-binding protein from bacteriophage T4 and related phages [D] . Boutemy, Laurence S. 2008

机译：5'至3'核酸外切酶与噬菌体T4和相关噬菌体的单链DNA结合蛋白之间的相互作用分析
6. Crystal structure of the phage T4 recombinase UvsX and its functional interaction with the T4 SF2 helicase UvsW [O] . Stefan Gajewski, Michael R. Webb, Vitold Galkin, -1

机译：噬菌体T4重组酶UVSX的晶体结构及其与T4 SF2 Helicase UVSW的功能相互作用
7. Crystal Structure of the Phage T4 Recombinase UvsX and Its Functional Interaction with the T4 SF2 Helicase UvsW [O] . Stefan Gajewski, Michael R. Webb, Vitold Galkin, 2011

机译：噬菌体T4重组酶UVSX的晶体结构及其与T4 SF2 Helicase UVSW的功能相互作用
8. Structure of the Phage T4 Tail Fiber Angle [R] . Goldberg, E. 2002

机译：噬菌体T4尾纤维角度的结构

Crystal structure of the phage T4 recombinase UvsX and its functional interaction with the T4 SF2 helicase UvsW.

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