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首页> 外文期刊>Journal of Molecular Biology >Mass spectrometry - From peripheral proteins to membrane motors
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Mass spectrometry - From peripheral proteins to membrane motors

机译：质谱-从外围蛋白质到膜驱动器

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That membrane protein complexes could survive in the gas phase had always seemed impossible. The lack of chargeable residues, high hydrophobicity, and poor solubility and the vast excess of detergent contributed to the view that it would not be possible to obtain mass spectra of intact membrane complexes. With the recent success in recording mass spectra of these complexes, first from recombinant sources and later from the cellular environment, many surprising properties of these gas phase membrane complexes have been revealed. The first of these was that the interactions between membrane and soluble subunits could survive in vacuum, without detergent molecules adhering to the complex. The second unexpected feature was that their hydrophobicity and, consequently, lower charge state did not preclude ionization. The final surprising finding was that these gas phase membrane complexes carry with them lipids, bound specifically in subunit interfaces. This provides us with an opportunity to distinguish annular lipids that surround the membrane complexes, from structural lipids that have a role in maintaining structure and subunit interactions. In this perspective, we track these developments and suggest explanations for the various discoveries made during this research.
机译：膜蛋白复合物可以在气相中生存似乎一直是不可能的。缺乏带电荷的残基，高疏水性和差的溶解性以及大量过量的去污剂导致了这样的观点，即不可能获得完整的膜络合物的质谱。随着最近成功地记录了这些复合物的质谱图，首先是从重组来源，后来又从细胞环境中记录了这些气相膜复合物的许多令人惊讶的特性。其中第一个是膜和可溶性亚基之间的相互作用可以在真空中幸存，而洗涤剂分子不会附着在复合物中。第二个出乎意料的特征是它们的疏水性以及因此较低的电荷状态不会排除电离。最终令人惊讶的发现是这些气相膜复合物带有脂质，这些脂质特异性地结合在亚基界面上。这为我们提供了一个机会，可以将膜复合物周围的环状脂质与在维持结构和亚基相互作用中起作用的结构脂质区分开来。从这个角度出发，我们跟踪这些进展并提出对本研究中发现的各种发现的解释。

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