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首页> 外文期刊>Journal of Molecular Biology >Trashing of single-stranded dna generated during processing of arrested replication fork in E coli
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Trashing of single-stranded dna generated during processing of arrested replication fork in E coli

机译：在大肠杆菌中捕获的复制叉加工过程中产生的单链dna破坏

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摘要

We analyzed formation of single-stranded DNA (ssDNA) related to SOS induction in nalidixilate (Nal)-treated Escherichia coli, using immunofluorescence microscopy accompanied by computer analysis. We found enhancement of both ssDNA concentrations and cells having ssDNA foci that often localized around cellpoles. Analyzing several mutants deficient in DNA repair or replication, we found, after Nal treatment, that recN, recA, uvrD and dnaB failed to increase ssDNA concentration and that recG and particularly ruvA only partially enhanced it. In Nal-treated recB mutant, despite its failure in SOS induction, ssDNA foci positive cells increased with a slight enhancement of its concentration. These observations suggest the existence of a cellular process that sequesters genotoxic ssDNA as inert form, offering a new concept for SOS suppressor genes action.
机译：我们使用免疫荧光显微镜和计算机分析，分析了纳迪西酯（Nal）处理的大肠杆菌中与SOS诱导相关的单链DNA（ssDNA）的形成。我们发现ssDNA浓度和具有ssDNA病灶（通常位于细胞极周围）的细胞均得到增强。分析了几种DNA修复或复制不足的突变体，我们发现在Nal处理后，recN，recA，uvrD和dnaB无法增加ssDNA的浓度，recG尤​​其是ruvA只能部分增强它的浓度。在Nal处理的recB突变体中，尽管其无法成功诱导SOS，但ssDNA病灶阳性细胞却随着其浓度的轻微增加而增加。这些观察结果表明，存在以惰性形式隔离遗传毒性ssDNA的细胞过程，为SOS抑制基因的作用提供了新的概念。

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