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首页> 外文期刊>Journal of Molecular Biology >Programming microbes using pulse width modulation of optical signals
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Programming microbes using pulse width modulation of optical signals

机译：使用光信号的脉冲宽度调制对微生物编程

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Cells transmit and receive information via signalling pathways. A number of studies have revealed that information is encoded in the temporal dynamics of these pathways and has highlighted how pathway architecture can influence the propagation of signals in time and space. The functional properties of pathway architecture can also be exploited by synthetic biologists to enable precise control of cellular physiology. Here, we characterised the response of a bacterial light-responsive, two-component system to oscillating signals of varying frequencies. We found that the system acted as a low-pass filter, able to respond to low-frequency oscillations and unable to respond to high-frequency oscillations. We then demonstrate that the low-pass filtering behavior can be exploited to enable precise control of gene expression using a strategy termed pulse width modulation (PWM). PWM is a common strategy used in electronics for information encoding that converts a series of digital input signals to an analog response. We further show how the PWM strategy extends the utility of bacterial optogenetic control, allowing the fine-tuning of expression levels, programming of temporal dynamics, and control of microbial physiology via manipulation of a metabolic enzyme.
机译：细胞通过信号通路发送和接收信息。大量研究表明，信息被编码在这些路径的时间动态中，并突出显示了路径结构如何影响信号在时间和空间中的传播。合成生物学家还可以利用途径构架的功能特性来精确控制细胞生理。在这里，我们表征了细菌光响应性两组分系统对不同频率的振荡信号的响应。我们发现该系统充当低通滤波器，能够响应低频振荡，而不能响应高频振荡。然后，我们证明了可以利用低通滤波行为来使用称为脉冲宽度调制（PWM）的策略精确控制基因表达。 PWM是电子技术中用于信息编码的一种常见策略，该信息编码将一系列数字输入信号转换为模拟响应。我们进一步展示了PWM策略如何扩展细菌光遗传学控制的实用性，允许表达水平的微调，时间动态的编程以及通过代谢酶的控制来控制微生物生理。

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