• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Molecular Biology >Molecular Dissection of the Interface between the Type VI Secretion TssM Cytoplasmic Domain and the TssG Baseplate Component
【24h】

Molecular Dissection of the Interface between the Type VI Secretion TssM Cytoplasmic Domain and the TssG Baseplate Component

机译：VI型分泌TssM细胞质结构域和TssG基板组件之间界面的分子解剖

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The type VI secretion system (T6SS) is a multiprotein complex that catalyses toxin secretion through the bacterial cell envelope of various Gram-negative bacteria including important human pathogens. This machine uses a bacteriophage-like contractile tail to puncture the prey cell and inject harmful toxins. The T6SS tail comprises an inner tube capped by the cell-puncturing spike and wrapped by the contractile sheath. This structure is built on an assembly platform, the baseplate, which is anchored to the bacterial cell envelope by the TssJLM membrane complex (MC). This MC serves as both a tail docking station and a channel for the passage of the inner tube. The TssM transmembrane protein is a key component of the MC as it connects the inner and outer membranes. In this study, we define the TssM topology, highlighting a large but poorly studied 35-kDa cytoplasmic domain, TssM(Cyto), located between two transmembrane segments. Protein-protein interaction assays further show that TssM(Cyto) oligomerises and makes contacts with several baseplate components. Using computer predictions, we delineate two subdomains in TssM(Cyto), including a nucleotide triphosphatase (NTPase) domain, followed by a 110-aa extension. Finally, site-directed mutagenesis coupled to functional assays reveals the contribution of these subdomains and conserved motifs to the interaction with T6SS partners and to the function of the secretion apparatus. (C) 2016 Elsevier Ltd. All rights reserved.
机译：VI型分泌系统（T6SS）是一种多蛋白复合物，可通过各种革兰氏阴性细菌（包括重要的人类病原体）通过细菌细胞包膜催化毒素分泌。该机器使用类似噬菌体的可收缩尾巴刺穿猎物细胞并注入有害毒素。 T6SS尾部包括一个内管，该内管被细胞穿刺钉覆盖，并被可收缩的鞘包裹。这种结构建立在组装平台即底板上，该底板通过TssJLM膜复合物（MC）固定在细菌细胞包膜上。该MC既用作尾部对接站，又用作内管通过的通道。 TssM跨膜蛋白是MC的关键组成部分，因为它连接着内膜和外膜。在这项研究中，我们定义了TssM拓扑结构，突出了位于两个跨膜段之间的一个大型但研究较少的35 kDa胞质域TssM（Cyto）。蛋白质-蛋白质相互作用测定进一步显示，TssM（Cyto）寡聚并与数个基板组件接触。使用计算机预测，我们描绘了TssM（Cyto）中的两个子域，包括核苷酸三磷酸酶（NTPase）域，然后是110-aa延伸。最后，结合功能测定的定点诱变揭示了这些亚结构域和保守基序对与T6SS伴侣相互作用以及对分泌装置功能的贡献。 （C）2016 Elsevier Ltd.保留所有权利。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号