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首页> 外文期刊>Journal of Molecular Biology >Interrelationship between cytoplasmic retroviral Gag concentration and Gag-membrane association.
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Interrelationship between cytoplasmic retroviral Gag concentration and Gag-membrane association.

机译：细胞质逆转录病毒Gag浓度与Gag-膜结合之间的相互关系。

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摘要

The early events in the retrovirus assembly pathway, particularly the timing and nature of Gag translocation from the site of protein translation to the inner leaflet of the plasma membrane, are poorly understood. We have investigated the interrelationship between cytoplasmic Gag concentration and plasma membrane association using complementary live-cell biophysical fluorescence techniques in real time with both human T-cell leukemia virus type 1 (HTLV-1) and human immunodeficiency virus type 1 (HIV-1) Gag proteins. In particular, dual-color, z-scan fluorescence fluctuation spectroscopy in conjunction with total internal reflection fluorescence and conventional, epi-illumination imaging were utilized. Our results demonstrate that HTLV-1 Gag is capable of membrane targeting and particle assembly at low (i.e., nanomolar) cytoplasmic concentrations and that there is a critical threshold concentration (approaching micromolar) prior to the observation of HIV-1 Gag associated with the plasma membrane. These observations imply fundamental differences between HIV-1 and HTLV-1 Gag trafficking and membrane association.
机译：逆转录病毒装配途径中的早期事件，特别是Gag从蛋白质翻译位点到质膜内小叶的移位的时间和性质，了解得很少。我们已经研究了使用互补的活细胞生物物理荧光技术与人类T细胞白血病病毒1型（HTLV-1）和人类免疫缺陷病毒1型（HIV-1）实时进行的细胞质Gag浓度与质膜结合之间的相互关系Gag蛋白。尤其是，采用了双色z扫描荧光波动光谱技术以及全内反射荧光技术和常规的落射照明成像技术。我们的结果表明，HTLV-1 Gag能够在低（即纳摩尔）细胞质浓度下进行膜靶向和颗粒组装，并且在观察与血浆相关的HIV-1 Gag之前存在临界阈值浓度（接近微摩尔）膜。这些观察结果暗示了HIV-1和HTLV-1 Gag贩运与膜结合之间的根本差异。

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