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首页> 外文期刊>Journal of Molecular Biology >Adaptive Remodelling by FliN in the Bacterial Rotary Motor
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Adaptive Remodelling by FliN in the Bacterial Rotary Motor

机译：FliN在细菌旋转电机中的自适应重塑

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Sensory adaptation in the Escherichia coli chemosensory pathway has been the subject of interest for decades, with investigation focusing on the receptors that process extracellular inputs. Recent studies demonstrate that the flagellar motors responsible for cell locomotion also play a role, adding or subtracting FliM subunits to maximise sensitivity to pathway signals. It is difficult to reconcile this FliM remodelling with the observation that partner FliN subunits are relatively static fixtures in the motor. By fusing a fluorescent protein internally to FliN, we show that there is in fact significant FliN remodelling. The kinetics and stoichiometry of FliN in steady state and in adapting motors are investigated and found to match the behaviour of FliM in all respects except for timescale where FliN rates are about 4 times slower. We notice that motor adaptation is slower in the presence of the fluorescent protein, indicating a possible source for the difference. The behaviour of FliM and FliN is consistent with a kinetic and stoichiometric model that contradicts the traditional view of a packed, rigid motor architecture.
机译：数十年来，大肠杆菌化学感应途径中的感觉适应一直是人们关注的主题，研究重点是处理细胞外输入的受体。最近的研究表明，负责细胞运动的鞭毛马达也起着一定的作用，增加或减少FliM亚基，以最大化对途径信号的敏感性。由于伙伴FliN亚基是电动机中相对静态的固定装置，因此很难协调这种FliM重塑。通过将荧光蛋白内部融合到FliN，我们显示出实际上存在显着的FliN重塑。研究了FliN在稳态和自适应电动机中的动力学和化学计量，发现除了FliN速率慢约4倍的时标外，FliN在所有方面都与FliM的行为相匹配。我们注意到在荧光蛋白的存在下运动适应较慢，这表明差异的可能来源。 FliM和FliN的行为与动力学和化学计量模型相一致，该模型与传统的紧凑，刚性电机架构的观点相矛盾。

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