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首页> 外文期刊>Journal of Molecular Biology >The plant host can affect the encapsidation of brome mosaic virus (BMV) RNA: BMV virions are surprisingly heterogeneous
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The plant host can affect the encapsidation of brome mosaic virus (BMV) RNA: BMV virions are surprisingly heterogeneous

机译：植物宿主可以影响溴化花叶病毒（BMV）RNA的衣壳化：BMV病毒体出奇地异质

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摘要

Brome mosaic virus (BMV) packages its genomic and subgenomic RNAs into three separate viral particles. BMV purified from barley, wheat, and tobacco have distinct relative abundances of the encapsidated RNAs. We seek to identify the basis for the host-dependent differences in viral RNA encapsidation. Sequencing of the viral RNAs revealed recombination events in the 3′ untranslated region of RNA1 of BMV purified from barley and wheat, but not from tobacco. However, the relative amounts of the BMV RNAs that accumulated in barley and wheat are similar and RNA accumulation is not sufficient to account for the difference in RNA encapsidation. Virions purified from barley and wheat were found to differ in their isoelectric points, resistance to proteolysis, and contacts between the capsid residues and the RNA. Mass spectrometric analyses revealed that virions from the three hosts had different post-translational modifications that should impact the physiochemical properties of the virions. Another major source of variation in RNA encapsidation was due to the purification of BMV particles to homogeneity. Highly enriched BMV present in lysates had a surprising range of sizes, buoyant densities, and distinct relative amounts of encapsidated RNAs. These results show that the encapsidated BMV RNAs reflect a combination of host effects on the physiochemical properties of the viral capsids and the enrichment of a subset of virions. The previously unexpected heterogeneity in BMV should influence the timing of the infection and also the host innate immune responses.
机译：Brome花叶病毒（BMV）将其基因组和亚基因组RNA打包为三个单独的病毒颗粒。从大麦，小麦和烟草中提纯的BMV的衣壳RNA具有相对不同的相对丰度。我们寻求确定病毒RNA衣壳化的宿主依赖性差异的基础。病毒RNA的测序揭示了从大麦和小麦（而非烟草）纯化的BMV RNA1的3'非翻译区域中的重组事件。但是，在大麦和小麦中积累的BMV RNA的相对数量相似，并且RNA积累不足以解决RNA衣壳化的差异。发现从大麦和小麦中纯化的病毒颗粒在等电点，抗蛋白水解性和衣壳残基与RNA之间的接触方面不同。质谱分析显示，来自三个宿主的病毒体具有不同的翻译后修饰，应影响病毒体的理化性质。 RNA衣壳化的另一个主要变化来源是由于将BMV颗粒纯化至同质。裂解物中存在的高度富集的BMV具有令人惊讶的大小范围，浮力密度和衣壳化RNA的相对相对量。这些结果表明，衣壳化的BMV RNA反映了宿主对病毒衣壳的理化特性和一部分病毒体富集的综合作用。 BMV中以前未曾预料到的异质性将影响感染的时机，也将影响宿主固有的免疫反应。

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