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首页> 外文期刊>Journal of Molecular Biology >Functional Characterization of a Conserved Archaeal Viral Operon Revealing Single-Stranded DNA Binding, Annealing and Nuclease Activities
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Functional Characterization of a Conserved Archaeal Viral Operon Revealing Single-Stranded DNA Binding, Annealing and Nuclease Activities

机译：保守古细菌病毒操纵子揭示单链DNA结合，退火和核酸酶活性的功能表征。

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摘要

The majority of archaeal viral genes are of unknown function hindering our understanding of the virus life cycle and viral interactions with their host. Here, we first describe functional characterization of ORF131b (gp17) and ORF436 (gp18) of Sulfolobus islandicus rod-shaped virus 2 (SIRV2), both encoding proteins of unknown function and forming an operon with ORF207 (gp19). SIRV2 gp17 was found to be a single-stranded DNA (ssDNA) binding protein different in structure from all previously characterized ssDNA binding proteins. Mutagenesis of a few conserved basic residues suggested a U-shaped binding path for ssDNA. The recombinant gp18 showed an ssDNA annealing activity often associated with helicases and recombinases. To gain insight into the biological role of the entire operon, we characterized SIRV2 gp19 and showed it to possess a 5'-> 3' ssDNA exonuclease activity, in addition to the previously demonstrated ssDNA endonuclease activity. Further, in vitro pull-down assay demonstrated interactions between gp17 and gp18 and between gp18 and gp19 with the former being mediated by the intrinsically disordered C-terminus of gp17. The strand-displacement replication mode proposed previously for rudiviruses and the close interaction among the ssDNA binding, annealing and nuclease proteins strongly point to a role of the gene operon in genome maturation and/or DNA recombination that may function in viral DNA replication/repair. (C) 2015 Elsevier Ltd. All rights reserved.
机译：大多数古细菌病毒基因的功能未知，这阻碍了我们对病毒生命周期以及病毒与其宿主之间的相互作用的理解。在这里，我们首先描述Sulfolobus islandicus杆状病毒2（SIRV2）的ORF131b（gp17）和ORF436（gp18）的功能特征，二者均编码功能未知的蛋白并与ORF207（gp19）形成操纵子。发现SIRV2 gp17是单链DNA（ssDNA）结合蛋白，其结构不同于所有先前表征的ssDNA结合蛋白。几个保守的基本残基的诱变表明单链DNA的U形结合路径。重组gp18显示出通常与解旋酶和重组酶相关的ssDNA退火活性。为了深入了解整个操纵子的生物学作用，我们对SIRV2 gp19进行了表征，并显示除先前证明的ssDNA核酸内切酶活性外，它还具有5'-> 3'ssDNA核酸外切酶活性。此外，体外下拉测定法证明了gp17和gp18之间以及gp18和gp19之间的相互作用，而前者是由gp17的内在无序的C末端介导的。先前提出的针对rudiviruses的链置换复制模式以及ssDNA结合，退火和核酸酶蛋白之间的紧密相互作用，强烈指出了基因操纵子在基因组成熟和/或DNA重组中的作用，这可能在病毒DNA复制/修复中起作用。 （C）2015 Elsevier Ltd.保留所有权利。

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