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首页> 外文期刊>Journal of Molecular Biology >Sallimus and the Dynamics of Sarcomere Assembly in Drosophila Flight Muscles
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Sallimus and the Dynamics of Sarcomere Assembly in Drosophila Flight Muscles

机译：Sallimus和果蝇飞行肌中肌节装配的动力学。

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摘要

The Drosophila indirect flight muscles (IFM) can be used as a model for the study of sarcomere assembly. Here we use a transgenic line with a green fluorescent protein (GFP) exon inserted into the Z-disc-proximal portion of sallimus (Sls), also known as Drosophila titin, to observe sarcomere assembly during I FM development. Firstly, we confirm that Sls-GFP can be used in the heterozygote state without an obvious phenotype in IFM and other muscles. We then use Sls-GFP in the I FM to show that sarcomeres grow individually and uniformly throughout the fibre, growing linearly in length and in diameter. Finally, we show that limiting the amounts of Sls in the IFM using RNAi leads to sarcomeres with smaller Z-discs in their core, whilst the thick/thin filament lattice can form peripherally without a Z-disc. Thick filament preparations from those muscles show that although the Z-disc-containing core has thick filaments of a regular length, filaments from the peripheral lattice are longer and asymmetrical around the bare zone. Therefore, the Z-disc and Sls are required for thick filament length specification but not for the assembly of the thin/thick filament lattice. (C) 2015 Elsevier Ltd. All rights reserved.
机译：果蝇间接飞行肌肉（IFM）可以用作肌节组装研究的模型。在这里，我们使用转基因品系，将绿色荧光蛋白（GFP）外显子插入sallimus（Sls）的Z盘近端部分（也称为果蝇titin），以观察I FM发育过程中的肌节组装。首先，我们确认Sls-GFP可以杂合子状态使用，而在IFM和其他肌肉中没有明显的表型。然后，我们在I FM中使用Sls-GFP来显示肉瘤在整个纤维中均匀且均匀地生长，其长度和直径呈线性增长。最后，我们证明了使用RNAi限制IFM中Sls的数量会导致肉瘤的核心Z-disc较小，而粗/细丝晶格可以在外围形成Z-disc。来自那些肌肉的粗丝准备表明，尽管含Z盘的芯具有规则长度的粗丝，但是来自外围晶格的细丝却更长且围绕裸露区域不对称。因此，对于厚的灯丝长度规格，Z-disc和Sls是必需的，而对于薄/厚的灯丝晶格，则不需要。 （C）2015 Elsevier Ltd.保留所有权利。

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