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首页> 外文期刊>Journal of Molecular Biology >Regions 1.2 and 3.2 of the RNA Polymerase sigma Subunit Promote DNA Melting and Attenuate Action of the Antibiotic Lipiarmycin
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Regions 1.2 and 3.2 of the RNA Polymerase sigma Subunit Promote DNA Melting and Attenuate Action of the Antibiotic Lipiarmycin

机译：RNA聚合酶sigma亚基的区域1.2和3.2促进抗生素Lipiarmycin的DNA熔解和减弱作用

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摘要

Initiation of RNA synthesis by bacterial RNA polymerase (RNAP) requires melting of promoter DNA, which is nucleated by the a subunit during formation of the "open" promoter complex (RPo). The antibiotic lipiarmycin (Lpm) inhibits promoter melting by blocking access of the template DNA strand to the RNAP active-site cleft. Here we show that Escherichia coli RNAP holoenzymes containing either housekeeping sigma(70), with a deletion in the region 3.2, or the stationary phase sigma(s) subunits exhibited hypersensitivity to Lpm and increased cold sensitivity of RPo formation. Similar effects were produced by mutation located similar to 60 angstrom away from the Lpm binding site within sigma(70) region 1.2, controlling-10 promoter element recognition. Our data suggested that template strand single-stranded DNA competes with Lpm for binding to RNAP and that sigma(70) regions 1.2 and 3.2 attenuate Lpm action by promoting DNA duplex opening. (C) 2015 Elsevier Ltd. All rights reserved.
机译：通过细菌RNA聚合酶（RNAP）启动RNA合成需要融化启动子DNA，在“开放式”启动子复合体（RPo）形成过程中，该链被a亚基成核。抗生素lipiarmycin（Lpm）通过阻止模板DNA链进入RNAP活性位点裂口来抑制启动子融化。在这里我们显示，大肠杆菌RNAP全酶含有管家sigma（70），在区域3.2中缺失，或固定相sigma（s）亚基表现出对Lpm的超敏性和RPo形成的冷敏性。通过位于sigma（70）1.2区域内距Lpm结合位点60埃附近的突变产生相似的效果，控制10个启动子元件的识别。我们的数据表明模板链单链DNA与Lpm竞争与RNAP的结合，而sigma（70）区域1.2和3.2通过促进DNA双链体的开放来减弱Lpm的作用。 （C）2015 Elsevier Ltd.保留所有权利。

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