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首页> 外文期刊>Journal of Materials Chemistry: An Interdisciplinary Journal dealing with Synthesis, Structures, Properties and Applications of Materials, Particulary Those Associated with Advanced Technology >Preparation and characterization of uniformly sized molecularly imprinted polymers functionalized with core-shell magnetic nanoparticles for the recognition and enrichment of protein
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Preparation and characterization of uniformly sized molecularly imprinted polymers functionalized with core-shell magnetic nanoparticles for the recognition and enrichment of protein

机译：制备并表征尺寸均匀的分子印迹聚合物，该聚合物被核-壳磁性纳米颗粒官能化以识别和富集蛋白质

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摘要

A general method to prepare thin, molecularly imprinted polymer (MIP) coatings on magnetic Fe3O4 nanoparticles (NPs) with a uniform core-shell structure for the recognition and enrichment of protein was developed. Four proteins (bovine serum albumin (BSA, pI = 4.9), bovine hemoglobin (BHb, pI = 6.9), bovine pancreas ribonuclease A (RNase A, pI = 9.4) and lysozyme (Lyz, pI = 11.2)) with different isoelectric points were chosen as the templates. The magnetic protein-MIPs were synthesized by combining surface imprinting and sol-gel techniques. The morphology, adsorption and recognition properties of the magnetic molecularly imprinted NPs were investigated by transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD), and Fourier transform infrared (FT-IR) spectroscopy and through the use of a vibrating sample magnetometer (VSM). In comparison with the use of Lyz, BSA and RNase A as template proteins, BHb-imprinted Fe3O4 showed the best imprinting effect and the highest adsorption capacity among the four proteins. The as-prepared Fe3O4@BHb-MIPs NPs with a mean diameter of 230 nm were coated with an MIP shell that was 10 nm thick, which enabled the Fe3O4@BHb-MIPs to easily reach adsorption equilibrium. A high magnetic saturation value of 25.47 emu g~(-1) for Fe3O4@BHb-MIPs NPs was obtained, which endowed the adsorbent with the convenience of magnetic separation under an external magnetic field. The resultant Fe3O4@BHb-MIPs NPs could not only selectively extract a target protein from mixed proteins but also specifically capture the protein BHb from a real sample of bovine blood. In addition, different batches of magnetic MIPs showed good reproducibility and reusability for at least six repeated cycles.
机译：开发了一种在具有均匀核壳结构的磁性Fe3O4纳米颗粒（NPs）上制备薄的分子印迹聚合物（MIP）涂层的通用方法，该涂层用于识别和富集蛋白质。四种蛋白质（等电点不同）（牛血清白蛋白（BSA，pI = 4.9），牛血红蛋白（BHb，pI = 6.9），牛胰腺核糖核酸酶A（RNase A，pI = 9.4）和溶菌酶（Lyz，pI = 11.2））被选为模板。通过结合表面印迹和溶胶-凝胶技术合成了磁性蛋白质-MIP。通过透射电子显微镜（TEM），扫描电子显微镜（SEM），X射线衍射（XRD）和傅里叶变换红外（FT-IR）光谱研究了磁性分子印迹NP的形态，吸附和识别特性，并通过使用振动样品磁力计（VSM）。与使用Lyz，BSA和RNase A作为模板蛋白相比，BHb印迹的Fe3O4在四种蛋白中表现出最佳的印迹效果和最高的吸附能力。制备的平均直径为230 nm的Fe3O4 @ BHb-MIPs NPs涂有10 nm厚的MIP壳，这使Fe3O4 @ BHb-MIPs易于达到吸附平衡。 Fe3O4 @ BHb-MIPs NPs的磁饱和值为25.47 emu g〜（-1），这使得吸附剂具有在外部磁场下进行磁选的便利性。生成的Fe3O4 @ BHb-MIPs NPs不仅可以从混合蛋白中选择性地提取目标蛋白，而且可以从真实的牛血样本中特异性捕获BHb蛋白。此外，不同批次的磁性MIP在至少六个重复循环中均显示出良好的可重复性和可重复使用性。

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