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首页> 外文期刊>Journal of Materials Chemistry: An Interdisciplinary Journal dealing with Synthesis, Structures, Properties and Applications of Materials, Particulary Those Associated with Advanced Technology >Synthesis, characterization, cellular uptake and cytotoxicity of a multifunctional magnetic nanocomposite for the targeted delivery and controlled release of doxorubicin to cancer cells
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Synthesis, characterization, cellular uptake and cytotoxicity of a multifunctional magnetic nanocomposite for the targeted delivery and controlled release of doxorubicin to cancer cells

机译：多功能磁性纳米复合材料的合成，表征，细胞摄取和细胞毒性，用于将阿霉素靶向递送和控制释放至癌细胞

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The primary inadequacy of chemotherapeutic drugs is their relative non-specificity and potential side effects towards healthy tissue. To overcome this, in this study a novel drug delivery system, namely, carboxymethyl chitosan capped magnetic nanoparticle intercalated montmorillonite nanocomposites (CMCS-capped-MNP/MMT) was developed and characterized by transmission electron microscopy, dynamic light scattering, Fourier transform infrared spectroscopy, X-ray diffraction, small-angle X-ray scattering, zeta potential analysis and a superconducting quantum interference device. The optimum pH value for the encapsulation of doxorubicin (DOX) into CMCS-capped-MNP/MMT was examined. The controlled release behavior of DOX was examined at 5.0 and 7.4 pH. The release rate of the loaded drug molecules was slow at pH 7.4 but increased significantly at acidic pH 5.0. The cytotoxicity of DOX-loaded-CMCS-capped-MNP/MMT towards MCF-7 cancer cells was investigated. The results showed that DOX-loaded-CMCS-capped-MNP/MMT retained significant antitumor activities. The cellular uptake of the fluorescent coumarin 6-loaded CMCS-capped-MNP/MMT on HeLa cells was analyzed with confocal laser scanning microscopy (CLSM) and flow cytometry and the results showed that MMT enhanced the cellular uptake efficiency. The effects of DOX and DOX-loaded-CMCS-capped-MNP/MMT on H9c2 cell death were investigated by using a microplate reader. The heating characteristics of the magnetic nanocomposites were investigated in a high frequency alternating magnetic gradient; a stable maximum temperature of 45 °C was successfully achieved within 40 min. The study demonstrated that CMCS-capped-MNP/MMT is not only a good delivery system for DOX but also appears to be a promising strategy for protecting against oxidative injury observed in DOX induced cardiotoxicity.
机译：化疗药物的主要不足之处是其相对非特异性以及对健康组织的潜在副作用。为了克服这个问题，在这项研究中，开发了一种新型的药物递送系统，即羧甲基壳聚糖包覆的磁性纳米粒子插层蒙脱土纳米复合材料（CMCS-capted-MNP / MMT），并通过透射电子显微镜，动态光散射，傅里叶变换红外光谱， X射线衍射，小角度X射线散射，ζ电位分析和超导量子干涉装置。检查了将阿霉素（DOX）封装到CMCS封端的MNP / MMT中的最佳pH值。在5.0和7.4 pH下检查DOX的控释行为。负载的药物分子的释放速率在pH 7.4时缓慢，但在酸性pH 5.0时显着增加。研究了DOX负载的CMCS封端的MNP / MMT对MCF-7癌细胞的细胞毒性。结果表明载有DOX的CMCS封端的MNP / MMT保留了显着的抗肿瘤活性。用共聚焦激光扫描显微镜（CLSM）和流式细胞仪分析了荧光香豆素6负载的CMCS封端的MNP / MMT在HeLa细胞上的细胞摄取，结果表明MMT增强了细胞摄取效率。通过使用酶标仪读取DOX和DOX加载的CMCS封端的MNP / MMT对H9c2细胞死亡的影响。在高频交变磁梯度下研究了磁性纳米复合材料的加热特性。 40分钟内成功达到45°C的稳定最高温度。该研究表明，CMCS封端的MNP / MMT不仅是DOX的良好递送系统，而且似乎是一种有前景的策略，可防止在DOX诱导的心脏毒性中观察到氧化损伤。

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