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首页> 外文期刊>Journal of Neurochemistry: Offical Journal of the International Society for Neurochemistry >The effect of phosphorylation on arrestin-rhodopsin interaction in the squid visual system
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The effect of phosphorylation on arrestin-rhodopsin interaction in the squid visual system

机译：鱿鱼视觉系统中磷酸化对抑制素-视紫红质相互作用的影响

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摘要

Invertebrate visual opsins are G protein-coupled receptors coupled to retinoid chromophores that isomerize reversibly between inactive rhodopsin and active metarhodopsin upon absorption of photons of light. The squid visual system has an arrestin protein that binds to metarhodopsin to block signaling to G(q) and activation of phospholipase C. Squid rhodopsin kinase (SQRK) can phosphorylate both metarhodopsin and arrestin, a dual role that is unique among the G protein-coupled receptor kinases. The sites and role of arrestin phosphorylation by SQRK were investigated here using recombinant proteins. Arrestin was phosphorylated on serine 392 and serine 397 in the C-terminus. Unphosphorylated arrestin bound to metarhodopsin and phosphorylated metarhodopsin with similar high affinities (K-d 33 and 21 nM respectively), while phosphorylation of arrestin reduced the affinity 3- to 5-fold (K-d 104 nM). Phosphorylation of metarhodopsin slightly increased the dissociation of arrestin observed during a 1 hour incubation. Together these studies suggest a unique role for SQRK in phosphorylating both receptor and arrestin and inhibiting the binding of these two proteins in the squid visual system.
机译：无脊椎动物视觉视蛋白是与类视色素发色团偶联的G蛋白偶联受体，在吸收光子后可在非活性视紫红质和活性视紫红质之间发生可逆异构化。鱿鱼视觉系统的视紫红质抑制蛋白与金属视紫红质结合，阻断信号传导至G（q）和激活磷脂酶C。鱿鱼视紫红质激酶（SQRK）可以磷酸化视紫红质和视紫红质，这是G蛋白独特的双重作用，偶联受体激酶。在这里使用重组蛋白研究了SQRK抑制蛋白磷酸化的位点和作用。 Arrestin在C端的392和397丝氨酸上被磷酸化。未磷酸化的抑制蛋白以相似的高亲和力（分别为K-d 33和21 nM）结合到视紫红质和磷酸化的视紫红质上，而抑制蛋白的磷酸化将亲和力降低了3到5倍（K-d 104 nM）。在1小时的孵育过程中，去甲视紫红质的磷酸化稍微增加了抑制蛋白的解离。这些研究共同表明，SQRK在使受体和抑制蛋白磷酸化并抑制鱿鱼视觉系统中这两种蛋白的结合方面具有独特作用。

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