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首页> 外文期刊>Journal of Neurochemistry: Offical Journal of the International Society for Neurochemistry >1,8-and 1,4-cineole enhance spontaneous excitatory transmission by activating different types of transient receptor potential channels in the rat spinal substantia gelatinosa
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1,8-and 1,4-cineole enhance spontaneous excitatory transmission by activating different types of transient receptor potential channels in the rat spinal substantia gelatinosa

机译：1,8-和1,4-桉树脑通过激活大鼠脊髓明胶样物质中不同类型的瞬时受体电位通道增强自发性兴奋性传递

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摘要

Although transient receptor potential (TRP) channels expressed in the spinal substantia gelatinosa play a role in modulating nociceptive transmission, their properties have not been fully examined yet. In order to address this issue, the effects of 1,8-cineole and its stereoisomer 1,4-cineole on excitatory transmission were examined by applying the whole-cell patch-clamp technique to substantia gelatinosa neurons in adult rat spinal cord slices. Miniature excitatory postsynaptic current frequency was increased by 1,8- and 1,4-cineole. The cineole activities were repeated and resistant to voltage-gated Na+-channel blocker tetrodotoxin. The 1,8-cineole activity was inhibited by TRP ankyrin-1 (TRPA1) antagonists (HC-030031 and mecamylamine) but not TRP vanilloid-1 (TRPV1) antagonists (capsazepine and SB-366791), whereas the 1,4-cineole activity was depressed by the TRPV1 but not TRPA1 antagonists. Although 1,8- and 1,4-cineole reportedly activate TRP melastatin-8 (TRPM8) channels, their activities were unaffected by TRPM8 antagonist 4-(3-chloro-2-pyridinyl)-N-[4-(1,1-dimethylethyl)phenyl]-1-piperazinecarboxamide. Monosynaptically evoked C-fiber, but not A-fiber excitatory postsynaptic current amplitude, was reduced by 1,8- and 1,4-cineole. These results indicate that 1,8- and 1,4-cineole increase spontaneous l-glutamate release from nerve terminals by activating TRPA1 and TRPV1 channels, respectively, while inhibiting C-fiber but not A-fiber evoked l-glutamate release. This difference between 1,8- and 1,4-cineole may serve to know the properties of TRP channels located in the central terminals of primary-afferent neurons.
机译：尽管在脊髓明胶体中表达的瞬时受体电位（TRP）通道在调节伤害性传递中起作用，但其性质尚未得到充分检查。为了解决这个问题，通过将全细胞膜片钳技术应用于成年大鼠脊髓切片中的明胶质神经元，研究了1,8-桉树脑及其立体异构体1,4-桉树脑对兴奋性传递的影响。微型兴奋性突触后电流频率增加了1,8-和1,4-桉树脑。重复了桉树脑活性，并且对电压门控的Na +通道阻滞剂河豚毒素具有抗性。 1,8-桉树脑活性被TRP锚蛋白1（TRPA1）拮抗剂（HC-030031和美甲胺）抑制，但未被TRP香草-1（TRPV1）拮抗剂（capsazepine和SB-366791）抑制，而1,4-桉树脑TRPV1抑制了活性，但TRPA1拮抗剂没有抑制。虽然据报道1,8-和1,4-桉树脑激活了TRP melastatin-8（TRPM8）通道，但它们的活性不受TRPM8拮抗剂4-（3-氯-2-吡啶基）-N- [4-（1,1）的影响-二甲基乙基）苯基] -1-哌嗪甲酰胺。单突触诱发的C纤维，而不是A纤维兴奋性突触后电流幅度降低了1,8-和1,4-桉树脑。这些结果表明1,8-和1,4-桉树脑分别通过激活TRPA1和TRPV1通道增加自神经末梢自发的l-谷氨酸的释放，同时抑制C-纤维而不是A-纤维诱发的l-谷氨酸的释放。 1,8-和1,4-桉树脑之间的这种差异可能有助于了解位于原发性神经元中央末端的TRP通道的特性。

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