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首页> 外文期刊>Journal of Neurophysiology >Large-scale identification of proteins involved in the development of a sexually dimorphic behavior
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Large-scale identification of proteins involved in the development of a sexually dimorphic behavior

机译：大规模鉴定涉及性二态行为发展的蛋白质

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Sexually dimorphic behaviors develop under the influence of sex steroids, which induce reversible changes in the underlying neural network of the brain. However, little is known about the proteins that mediate these activational effects of sex steroids. Here, we used a proteomics approach for large-scale identification of proteins involved in the development of a sexually dimorphic behavior, the electric organ discharge of brown ghost knifefish, Apteronotus leptorhynchus. In this weakly electric fish, the discharge frequency is controlled by the medullary pacemaker nucleus and is higher in males than in females. After lowering the discharge frequency by chronic administration of beta-estradiol, 2-dimensional difference gel electrophoresis revealed 62 proteins spots in tissue samples from the pacemaker nucleus that exhibited significant changes in abundance of > 1.5-fold. The 20 identified protein spots indicated, among others, a potential involvement of astrocytes in the establishment of the behavioral dimorphism. Indeed, immunohistochemical analysis demonstrated higher expression of the astrocytic marker protein GFAP and increased gap-junction coupling between astrocytes in females compared with males. We hypothesize that changes in the size of the glial syncytium, glial coupling, and/or number of glia-specific potassium channels lead to alterations in the firing frequency of the pacemaker nucleus via a mechanism mediating the uptake of extracellular potassium ions from the extracellular space.
机译：性双态行为是在性类固醇的影响下发展的，这会导致潜在的大脑神经网络发生可逆变化。然而，对于介导性类固醇的这些激活作用的蛋白质知之甚少。在这里，我们使用蛋白质组学方法大规模鉴定参与性二态行为，褐色鬼刀鱼的电器官放电，Apteronotus leptorhynchus的发育的蛋白质。在这种弱电鱼中，放电频率由髓核起搏器核控制，雄性高于雌性。在通过长期施用β-雌二醇降低放电频率后，二维差异凝胶电泳显示了来自起搏器核的组织样品中的62个蛋白点，其丰度变化显着> 1.5倍。鉴定出的20个蛋白质斑点表明，星形胶质细胞可能参与了行为二态性的建立。确实，免疫组化分析表明，与男性相比，雌性中星形细胞标记蛋白GFAP的表达更高，星形胶质细胞之间的间隙连接偶联增加。我们假设神经胶质合胞体的大小，神经胶质结合和/或神经胶质特异性钾通道数量的变化通过介导细胞外空间摄取细胞外钾离子的机制导致起搏器核的发射频率改变。 。

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