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首页> 外文期刊>Journal of Neurophysiology >Anesthetic regimes modulate the temporal dynamics of local field potential in the mouse olfactory bulb
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Anesthetic regimes modulate the temporal dynamics of local field potential in the mouse olfactory bulb

机译：麻醉机制调节小鼠嗅球中局部场电位的时间动态

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Anesthetized preparations have been widely used to study odor-induced temporal dynamics in the olfactory bulb. Although numerous recent data of single-cell recording or imaging in the olfactory bulb have employed ketamine cocktails, their effects on networks activities are still poorly understood, and odor-induced oscillations of the local field potential have not been characterized under these anesthetics. Our study aimed at describing the impact of two ketamine cocktails on oscillations and comparing them to awake condition. Anesthesia was induced by injection of a cocktail of ketamine, an antagonist of the Af-methyl-D-aspartate receptors, combined with one agonist of alpha2-adrenergic receptors, xyla-zine (low affinity) or medetomidine (high affinity). Spontaneous and odor-induced activities were examined in anesthetized and awake conditions, in the same mice chronically implanted with an electrode in the main olfactory bulb. The overall dynamic pattern of oscillations under the two ketamine cocktails resembles that of the awake state. Ongoing activity is characterized by gamma bursts (>60 Hz) locked on respiration and beta (15-40 Hz) power increases during odor stimulation. However, anesthesia decreases local field potential power and leads to a strong frequency shift of gamma oscillations from 60-90 Hz to 100-130 Hz. We conclude that similarities between oscillations in anesthetized and awake states make cocktails of ketamine with one alpha2-agonist suitable for the recordings of local field potential to study processing in the early stages of the olfactory system.
机译：麻醉制剂已被广泛用于研究嗅球中气味引起的时间动态。尽管最近有许多关于嗅球中单细胞记录或成像的数据已经使用了氯胺酮混合物，但是它们对网络活动的影响仍然知之甚少，并且在这些麻醉剂下还没有表征气味引起的局部场电位振荡。我们的研究旨在描述两种氯胺酮鸡尾酒对振荡的影响，并将其与清醒状态进行比较。通过注射氯胺酮混合物来诱导麻醉，氯胺酮混合物是Af-甲基-D-天冬氨酸受体的拮抗剂，与α2-肾上腺素能受体的一种激动剂，木拉嗪（低亲和力）或美托咪定（高亲和力）结合使用。在麻醉和清醒条件下，在长期在主嗅球中植入电极的同一只小鼠中，检查了自发性和气味诱导的活性。在两个氯胺酮混合物下振荡的整体动态模式类似于清醒状态。持续进行的活动的特征是在呼吸时锁定了伽马脉冲（> 60 Hz），在气味刺激过程中β（15-40 Hz）功率增加。但是，麻醉会降低局部场电势，并导致伽玛振荡从60-90 Hz强烈移至100-130 Hz。我们得出结论，麻醉状态和清醒状态之间的振荡相似性使氯胺酮与一种α2-激动剂的混合物适合于局部场电位的记录，以研究嗅觉系统的早期过程。

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