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首页> 外文期刊>Journal of Neurophysiology >Large-scale imaging of cortical dynamics during sensory perception and behavior
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Large-scale imaging of cortical dynamics during sensory perception and behavior

机译：感觉知觉和行为期间皮质动力学的大规模成像。

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Sensory-driven behaviors engage a cascade of cortical regions to process sensory input and generate motor output. To investigate the temporal dynamics of neural activity at this global scale, we have improved and integrated tools to perform functional imaging across large areas of cortex using a transgenic mouse expressing the genetically encoded calcium sensor GCaMP6s, together with a head-fixed visual discrimination behavior. This technique allows imaging of activity across the dorsal surface of cortex, with spatial resolution adequate to detect differential activity in local regions at least as small as 100 mu m. Imaging during an orientation discrimination task reveals a progression of activity in different cortical regions associated with different phases of the task. After cortex-wide patterns of activity are determined, we demonstrate the ability to select a region that displayed conspicuous responses for two-photon microscopy and find that activity in populations of individual neurons in that region correlates with locomotion in trained mice. We expect that this paradigm will be a useful probe of information flow and network processing in brain-wide circuits involved in many sensory and cognitive processes.
机译：感觉驱动行为使皮质区域级联，以处理感觉输入并产生运动输出。为了研究这种全球规模的神经活动的时间动态，我们已经改进和集成了工具，可以使用表达遗传编码的钙传感器GCaMP6s的转基因小鼠，并通过头部固定的视觉辨别行为，在大皮质区域执行功能成像。这种技术可以对整个皮质背表面的活动进行成像，其空间分辨率足以检测至少100微米的局部区域的差异活动。定向识别任务期间的成像显示与任务的不同阶段相关的不同皮质区域的活动进展。确定了整个大脑皮层的活动模式后，我们证明了选择显示双光子显微镜显着响应的区域的能力，并发现该区域中单个神经元群体的活动与受过训练的小鼠的运动有关。我们希望这种范例将成为涉及许多感觉和认知过程的全脑电路中信息流和网络处理的有用探究。

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