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首页> 外文期刊>Journal of Physics, D. Applied Physics: A Europhysics Journal >Spot size variation FCS in simulations of the 2D Ising model
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Spot size variation FCS in simulations of the 2D Ising model

机译：2D Ising模型仿真中的光斑尺寸变化FCS

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Spot variation fluorescence correlation spectroscopy (svFCS) was developed to study the movement and organization of single molecules in plasma membranes. This experimental technique varies the size of an illumination area while measuring correlations in time using standard fluorescence correlation methods. Frequently, this data is interpreted using the assumption that correlation measurements reflect the dynamics of single molecule motions, and not motions of the average composition. Here, we explore how svFCS measurements report on the dynamics of components diffusing within simulations of a 2D Ising model with a conserved order parameter. Simulated correlation functions report on both the fast dynamics of single component mobility and the slower dynamics of the average composition. Over a range of simulation conditions, a conventional svFCS analysis suggests the presence of anomalous diffusion even though single molecule motions are nearly Brownian in these simulations. This misinterpretation is most significant when the surface density of the fluorescent label is elevated, therefore we suggest future measurements be made over a range of tracer densities. Some simulation conditions reproduce qualitative features of published svFCS experimental data. Overall, this work emphasizes the need to probe membranes using multiple complimentary experimental methodologies in order to draw conclusions regarding the nature of spatial and dynamical heterogeneity in these systems.
机译：斑点变化荧光相关光谱法（svFCS）被开发来研究质膜中单个分子的运动和组织。该实验技术在使用标准荧光相关方法及时测量相关性的同时，改变了照明区域的大小。通常，此数据使用以下假设进行解释：相关性测量反映的是单分子运动的动力学，而不是平均组成的运动。在这里，我们探索svFCS测量如何报告具有保守阶数参数的2D Ising模型的模拟内扩散的组件动力学。模拟的相关函数报告了单组分迁移率的快速变化和平均组成的较慢变化。在一系列模拟条件下，常规svFCS分析表明存在异常扩散，即使在这些模拟中单分子运动接近布朗运动。当荧光标记的表面密度升高时，这种误解最为明显，因此我们建议将来在示踪剂密度范围内进行测量。一些模拟条件可再现已发布的svFCS实验数据的定性特征。总的来说，这项工作强调需要使用多种互补的实验方法来探测膜，以便得出关于这些系统中空间和动态异质性的结论。

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