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首页> 外文期刊>Cryo Letters >PROGRAMMED CELL DEATH AND NECROSIS DURING CRYOPREPARATIVE DRYING OF IN VITRO Eucalyptus grandis AXILLARY BUDS
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PROGRAMMED CELL DEATH AND NECROSIS DURING CRYOPREPARATIVE DRYING OF IN VITRO Eucalyptus grandis AXILLARY BUDS

机译：桉树腋芽低温复制干燥过程中的程序性细胞死亡和坏死

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In preparation for cryopreservation, Eucalyptus grandis in vitro axillary buds were dried over silica gel. Pretreatment of the buds with 5 mg 1~-1 ABA resulted in partial resistance to water loss (0.76 to 0.45 g g~-1 fresh mass basis) as compared with untreated buds (0.76 to 0.33 g g~-1) and was associated with the retention of viability (70 vs. 55%). The loss of viability of the dried buds was protracted over several days. Ultrastructural examination and vital staining demonstrated cellular and tissue responses to drying. The meristem appeared to withstand drying and 72 h of rehydration whilst the leaf primordia were destroyed immediately after drying. High reactive oxygen species (ROS) activity was associated with bud excision and drying. Caspase-3-like protease activity was detected after rehydration, thereby providing evidence that the dried buds, that had ultimately died, had undergone programmed cell death. ROS production is considered to be the trigger for programmed cell death.
机译：在准备冷冻保存时，将巨桉体外腋芽用硅胶干燥。与未处理的芽（0.76至0.33 gg〜-1）相比，用5 mg 1〜-1 ABA预处理芽对水分损失（0.76至0.45 gg〜-1新鲜质量基准）有部分抵抗力，并且与生存能力的保留（70％对55％）。干燥芽的活力丧失持续了几天。超微结构检查和活体染色显示细胞和组织对干燥的反应。分生组织似乎可以经受干燥和补液72小时，而干燥后立即破坏了原基。高活性氧（ROS）活性与芽切除和干燥有关。补液后检测到类似Caspase-3的蛋白酶活性，从而提供证据表明最终死亡的干燥芽已经历了程序性细胞死亡。 ROS的产生被认为是程序性细胞死亡的触发。

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