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首页> 外文期刊>International Journal of Biological Macromolecules: Structure, Function and Interactions >Hyaluronic acid production enhancement via genetically modification and culture medium optimization in Lactobacillus acidophilus
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Hyaluronic acid production enhancement via genetically modification and culture medium optimization in Lactobacillus acidophilus

机译：透明质酸生产增强通过乳杆菌嗜酸乳杆菌的遗传修饰和培养基优化

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摘要

Hyaluronic acid (HA) is a natural polymer with various molecular weights that specify multiple biological roles. Traditionally, HA is obtained from animal waste and conventional pathogenic streptococci. However, there are challenges in these processes such as the presence of exotoxins, hyaluronidase, and viral contamination. In order to reduce these problems, this study was conducted to produce HA using recombinant bacterium that is generally recognized as safe (GRAS), and thereafter increase production through experimental design. At first, some lactic acid bacteria were screened and evaluated for HA production. Accordingly, among the selected bacteria, Lactobacillus acidophilus PTCC1643 produced about 025 g HA/L in the 48th hour of cultivation, and was thus selected as an alternative host for heterologous HA production. An expression vector containing HA synthase genes was transformed into L acidophilus by electroporation. Consequently, HA production increased to 0.4 g/L Eventually, response surface method (RSM) was used, which increased HA production to 1.7 g/L. This is approximately 7-fold higher than that produced at first. The resulting HA was characterized by FTIR spectroscopy and its molecular weight was estimated using agarose gel electrophoresis. In conclusion, L acidophilus could be a safe, effective, and novel HA producer with industrial potential and commercial prospects. (C) 2018 Published by Elsevier B.V.
机译：透明质酸（HA）是与指定多个生物角色各种分子量天然聚合物。传统上，HA是从动物废物和常规致病链球菌获得。但是，也有在这些过程中的挑战，如外毒素，透明质酸酶的存在，和病毒污染。为了减少这些问题，本研究进行使用通常被认为是安全的（GRAS）重组细菌产生HA，并且此后增加产量通过实验设计。首先，某些乳酸菌进行了筛选并评价HA生产。因此，所选择的细菌中，嗜酸乳杆菌PTCC1643产生约025克HA / L培养中的第48小时，从而被选择作为替代宿主异源HA的生产。含HA合酶基因的表达载体通过电穿孔转化成L-嗜酸。因此，HA产量提高至0.4g / L最终，采用响应表面方法（RSM），从而增加了生产HA至1.7克/升。这是大约7倍，在第一产生更高。将所得的HA进行表征通过FTIR光谱和其分子量使用琼脂糖凝胶电泳估算。总之，嗜酸乳杆菌可能是一种安全，有效，和新颖的HA生产商，工业潜力和商业前景。 （c）2018由elestvier b.v出版。

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