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首页> 外文期刊>International Journal of Biological Macromolecules: Structure, Function and Interactions >Intensification in biological properties of chitosan after gamma-irradiation
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Intensification in biological properties of chitosan after gamma-irradiation

机译：γ-辐照后壳聚糖生物学性质的强化

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Chitosan, a functional biopolymer, was irradiated with 100 kGy gamma irradiation and used to access its physical, antioxidant, plant growth promoting and antimicrobial properties. The molecular weight of chitosan reduced to 82.2 kDa from 337.7 kDa after irradiation. UV-Vis spectroscopy and FTIR results revealed slight changes in chitosan skeleton after irradiation but the degree of acetylation of both chitosan was similar to 18%. DSC profile indicated a prominent decline in enthalpy and energy for phase transition, TGA indicated shift in decomposition temperature, while XRD analysis showed a reduction in chitosan crystallinity after irradiation. DPPH and ABTS radical scavenging activity of chitosan (2-10 mg/mL) enhanced significantly by 1.25-1.45 and 1.80-3.14 folds after irradiation. There was a considerable improvement in morphological parameters (number of leaves, nodes, height, fresh weight and dry weight) and biochemical parameters (chlorophyll, total soluble sugars and soluble proteins content) of in vitro potato plant in chitosan supplemented medium at 75 mg/L concentration than the control. The minimum inhibitory concentration of normal and irradiated chitosan for Alternaria spp. was 2500 and 2000 mg/L and for Fusarium spp. was 1750 and 1500 mg/L, respectively. IC50 value of normal and irradiated chitosan for Fusarium spp. was 1387.9 +/- 9.2 and 954.3 +/- 6.1 mg/L, and for Alternaria spp. was 1536.1 +/- 24.3 and 1416.8 +/- 3.5 mg/L, respectively. (C) 2019 Elsevier B.V. All rights reserved.
机译：壳聚糖，一种功能性生物聚合物，用100kGyγ辐射照射，用于进入其物理，抗氧化剂，植物生长促进和抗微生物性质。壳聚糖的分子量在照射后从337.7kDa降至82.2kDa。 UV-Vis光谱和FTIR结果显示壳聚糖骨架在照射后显露轻微变化，但壳聚糖的乙酰化程度类似于18％。 DSC轮廓表明相焓和能量进行相转移的突出下降，TGA表示分解温度的变化，而XRD分析显示在照射后的壳聚糖结晶度降低。壳聚糖（2-10mg / ml）的DPPH和ABTS自由基清除活性在辐照后显着增强1.25-1.45和1.80-3.14折叠。在壳聚糖补充培养基中的体外马铃薯植物的形态参数（叶片，节点，高度，新鲜重量和干重）和生物化学参数（叶绿素，总可溶性糖和可溶性糖蛋白含量）具有相当大的改善，在75毫克/ l浓度比控制。 alterararia spp的正常和辐照壳聚糖的最小抑制浓度。是2500和2000 mg / l，用于镰刀菌。分别为1750和1500毫克/升。镰刀菌SPP正常和辐照壳聚糖的IC50值。是1387.9 +/- 9.2和954.3 +/- 6.1 mg / l，以及alterararia spp。分别为1536.1 +/- 24.3和1416.8 +/- 3.5 mg / L. （c）2019 Elsevier B.v.保留所有权利。

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