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首页> 外文期刊>International Journal of Biological Macromolecules: Structure, Function and Interactions >Evaluation of sodium alginate for encapsulation-vitrification of testicular Leydig cells
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Evaluation of sodium alginate for encapsulation-vitrification of testicular Leydig cells

机译：睾丸卵黄细胞包封 - 抗藻酸钠的评价

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This study reports encapsulation-vitrification of Leydig cells. The Leydig cells were encapsulated in sodium alginate beads of different sizes and cryopreserved by vitrification or slow freezing. Physico-chemical characterization of beads was done by Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffraction (XRD), Fluorescence Recovery after Photobleaching (FRAP) and in vitro biodegradation study. Surface morphology of cryopreserved cell-encapsulated beads was evaluated by Environmental Scanning Electron Microscopy (E-SEM), encapsulation efficiency and viability of cells were assessed by Trypan blue assay, mitochondria! activity (MTT assay) and cytoplasmic esterase enzyme activity (FDA assay), respectively. Results showed that vitrification gives better results than slow freezing with respect to surface morphology as well as cell viability of the cell-encapsulated beads (86.94 +/- 2.20% vs. 67.94 +/- 2.30%; p < 0.05). Encapsulation of cells in small diameter beads (1.8 mm) gave a better cell proliferation rate than large (2.1 mm and 2.7 mm). There was a significant difference in the population doubling time (47.9 +/- 1.7 h vs. 67.1 +/- 25 h) and cell proliferation rate (0.50 +/- 0.24 vs. 036 +/- 024 per day) of vitrified-warmed cell encapsulated beads with different diameter (p < 0.05). Encapsualtion in sodium alginate beads is a promising method for cryopreservation of Leydig cells by slow freezing as well as vitrification. (C) 2020 Elsevier B.V. All rights reserved.
机译：本研究报告了Leydig细胞的包封 - 玻璃化。将Leydig细胞包封在不同尺寸的藻酸钠珠粒中，并通过玻璃化或缓慢冷冻保存。通过傅里叶变换红外光谱（FTIR），X射线衍射（XRD），光漂白（FRAP）和体外生物降解研究荧光回收来完成珠粒的物理化学表征。通过环境扫描电子显微镜（E-SEM）评估冷冻保存的细胞包封珠粒的表面形态，通过Trypan Blue测定，线粒体评估细胞的包封效率和存活率！活性（MTT测定）和细胞质酯酶酶活性（FDA测定）。结果表明，玻璃化优于对表面形态的慢冻结较好的结果，以及细胞包封珠的细胞活力（86.94 +/- 2.20％与67.94 +/- 2.30％; P <0.05）。小直径珠粒（1.8mm）中的细胞的封装给出了比大（2.1mm和2.7mm）更好的细胞增殖率。人口倍增时间有显着差异（47.9 +/- 1.7小时，67.1 +/- 25小时）和细胞增殖率（每天0.50 +/- 0.24与036 +/- 024）的玻璃化温暖细胞包封珠，不同直径（P <0.05）。藻酸钠珠粒中的包装是通过缓慢冷冻和玻璃化的Leydig细胞进行冷冻保存的有希望的方法。 （c）2020 Elsevier B.v.保留所有权利。

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