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首页> 外文期刊>International Journal of Biological Macromolecules: Structure, Function and Interactions >Green synthesized and characterized copper nanoparticles using various new plants extracts aggravate microbial cell membrane damage after interaction with lipopolysaccharide
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Green synthesized and characterized copper nanoparticles using various new plants extracts aggravate microbial cell membrane damage after interaction with lipopolysaccharide

机译：使用各种新植物的绿色合成和表征铜纳米粒子提取物在与脂多糖相互作用后加剧微生物细胞膜损伤

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摘要

In the present study, commercially available six plants leave extracts such as Eucalyptus camaldulensis, Azadirachta indica, Murraya koenigii, Avicennia marina, Rosa tubiginosa and Datum stramonium were utilized for the production of copper nanoparticles (CuNPs). The characterization of particles was performed by UV/Vis. TEM, SEM, EDX and FTIR spectroscopy. TEM images showed the creation of CuNPs having mean size ranged from 48 to 29 nm corresponding to different plant extracts. SEM analysis showed the formation of spherical form of NPs. FTIR spectroscopy verified the availability of phytochemical components as they serves the reducing, covering and stabilizing assistant of the CuNPs. Antimicrobial ability of NPs was performed against various clinical pathogenic strains by Oxford cup method. The synthesized NPs indicated potent antibacterial activity, with relatively low values of MIC between 15 and 60 mu g/mL. The antibacterial effect of each CuNPs was observed in the resulting order A. indica > D. stramonium > M. koenigii > R. rubiginosa > A. marina > E. camaldulensis. After 12 h exposure with A. indica synthesized CuNPs, the SEM images of S. typhi showed destruction of cell membrane and cell lysis was clearly observed after interaction with lipopolysaccharide. In conclusion, these obtained CuNPs could be precisely applied in treatment protocols without any covering or core-shell procedures. (C) 2020 Elsevier B.V. All rights reserved.
机译：在本研究中，市售的六种植物留下提取物，如桉树Camaldulensis，Azadirachta indica，Murraya Koenigii，Avicennia Marina，Rosa Tubiginosa和基准醚酸盐的生产用于生产铜纳米粒子（CUNP）。颗粒的表征通过UV / Vis进行。 TEM，SEM，EDX和FTIR光谱。 TEM图像显示，具有与不同植物提取物相对应的平均尺寸的CUNP的创建。 SEM分析显示NPS的球形形式。 FTIR光谱学验证了植物化学成分的可用性，因为它们是用于CUNP的还原，覆盖和稳定助剂。通过牛津杯法针对各种临床病原菌进行NPS的抗微生物能力。合成的NPS表示有效的抗菌活性，具有15至60μmg/ ml之间的MIC值相对较低。在得到的顺序A.籼氏蛋白> D.Stramonium> M.Koenigii> R.Rubiginosa> A.Marina> E. Camaldulensis中，观察到每个CUNP的抗菌效果。 12小时后用A. Indipa合成CUNP接触后，在与脂多糖相互作用后清楚地观察到S. Typhi的SEM图像显示细胞膜和细胞裂解。总之，这些获得的CUNP可以精确地应用于治疗方案，而无需任何覆盖或核心壳程序。 （c）2020 Elsevier B.v.保留所有权利。

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