• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>International Journal of Biological Macromolecules: Structure, Function and Interactions >Entrapment of surfactant modified lipase within zeolitic imidazolate framework (ZIF)-8
【24h】

Entrapment of surfactant modified lipase within zeolitic imidazolate framework (ZIF)-8

机译：沸石咪唑酯骨架内表面活性剂改性脂肪酶的抑制（ZIF）-8

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Mostly, enzyme activity is reduced after immobilization of enzyme within MOF due to unfavourable conformational changes occurred during the immobilization procedure. In this context, lipase was activated by surfactants (in order to get a highly active enzyme) followed by encapsulation within zeolitic imidazolate framework (ZIF)-8 via one-pot facile self-assembly method. The prepared lipase-SDS ZIF-8 exhibited 250% enhanced activity compared to native form. The prepared biocomposite was characterized and analysed by X-ray diffraction (XRD), Fourier transform infrared (FT-IR) and scanning electron microscopy (SEM). Thermo-stability was determined for prepared lipase-SDS ZIF-8 biocomposite in the range of 50-70 degrees C, which showed more than two-folds enhanced stability in terms of half-life. Further, immobilized lipase retained 76% of residual activity even after six repetitive cycles and, it showed 91% residual activity after twenty days of long term storage. Finally, lipase-SDS ZIF-8 was tested for chemical stability in polar denaturing solvents which showed excellent stability as compared to free lipase. (C) 2019 Elsevier B.V. All rights reserved.
机译：大多数情况下，由于在固定过程中发生不利的构象变化，在MOF内的固定后，减少酶活性。在此上下文中，脂肪酶是由表面活性剂激活（为了得到高活性的酶），接着加入沸石咪唑酯骨架（ZIF）内封装-8经由一锅煮容易自组装法。与本地形式相比，制备的脂肪酶-SDS ZIF-8表现出250％的增强活性。通过X射线衍射（XRD），傅里叶变换红外（FT-IR）和扫描电子显微镜（SEM）进行制备的生物复合材料并分析。在50-70℃的制备脂肪酶-SDS ZIF-8生物复合中测定热稳定性，其在50-70℃的范围内，其在半衰期方面显示出大于两倍的稳定性。此外，即使在六个重复循环后，固定化脂肪酶也保留了76％的残余活性，并且在长期储存的二十天后，它显示出91％的残余活性。最后，在极性变性溶剂中测试脂肪酶-SDS ZIF-8的化学稳定性，其与游离脂肪酶相比显示出优异的稳定性。 （c）2019 Elsevier B.v.保留所有权利。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号