首页> 外文期刊>International Journal of Biological Macromolecules: Structure, Function and Interactions >Scanning electron microscopy reveals deleterious effects of Moringa oleifera seed exuded proteins on root-knot nematode Meloidogyne incognita eggs
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Scanning electron microscopy reveals deleterious effects of Moringa oleifera seed exuded proteins on root-knot nematode Meloidogyne incognita eggs

机译:扫描电子显微镜揭示了Moringa Oleifera种子渗出蛋白质在根结Nematode Meloidogyne incognita鸡蛋中的有害影响

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摘要

Plant seeds can exudate active molecules with inhibitory effects against several soil pathogens, including nematodes. This study aimed to characterize and evaluate the nematicidal properties against Meloidogyne incognita of exuded proteins from Moringa oleifera seeds. M. oleifera seeds were soaked in distilled water, and exudates were harvested and analyzed for the presence of defense proteins and anthelmintic activity. Enzymatic assays revealed the existence of PR-proteins such as beta-1,3-glucanases (0.18 +/- 0.003 nkatal mg(-1) of protein), chitinases (0.22 +/- 0.004 nkatalmg(-1) of protein), proteases (261.30 +/- 6.405 AU mg(-1) of proteinmin(-1)), serine (190.30 +/- 5.574 IA mg(-1) of protein) and cysteine (231.70 +/- 0.923 IA mg(-1) of protein), protease inhibitors. The exuded proteins presented ovicidal activity and caused 100% mortality of second-stage juveniles (J2s). Scanning electronmicroscopy (SEM) revealed deleterious effects onM. incognita eggs, such as invaginations, cracks, scratched surface, and loss of internal content. These findings confirmthe presence of anthelmintic proteins inM. oleifera seed exudate, possibly involved in plant defense during seed germination. Besides this, the exuded proteins exhibited strong biotechnological potential for use in the biocontrol of M. incognita infections, which are responsible for millions of dollars in staple crop losses every year. (C) 2019 Elsevier B.V. All rights reserved.
机译:植物种子可以渗出具有抑制作用的活性分子,对几种土壤病原体,包括线虫。本研究旨在表征和评估来自Moringa Oleifera种子的渗出蛋白的Meloidogyne incognita的象征性质。辣木种子浸泡在蒸馏水中,收获的分泌物和防御蛋白和抗蠕虫活性的存在进行分析。酶测定揭示了诸如β-1,3-葡聚糖酶(0.18 +/- 0.003 nkatal mg(-1)蛋白),几丁酶(0.22 +/- 0.004 nkatalmg(-1)蛋白)的蛋白质),蛋白酶(proteinmin的261.30 +/- 6.405 AU毫克(-1)(-1)),丝氨酸(190.30 +/- 5.574 IA mg蛋白质(-1))和半胱氨酸(231.70 +/- 0.923 IA毫克(-1 )蛋白质),蛋白酶抑制剂。渗出的蛋白质呈现过阳蛋白活性并引起的二阶青少年(J2S)的100%死亡率。扫描电子镜(SEM)显示有害影响ONM。隐性蛋白,如入侵,裂缝,划痕表面,以及内容损失。这些发现证明了存在扁平蛋白蛋白INM。 oleifera种子渗出,可能参与种子萌发过程中的植物防御。除此之外,出现的蛋白质表现出在肌电池的生物控制中使用的强烈生物技术潜力,每年负责数百万美元的主食作物损失。 (c)2019 Elsevier B.v.保留所有权利。

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