• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>International Journal of Biological Macromolecules: Structure, Function and Interactions >Effects of phosphate ion concentration on in-vitro fibrillogenesis of sturgeon type I collagen
【24h】

Effects of phosphate ion concentration on in-vitro fibrillogenesis of sturgeon type I collagen

机译：磷酸离子浓度对鲟鱼I型胶原体外纤维发生的影响

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Nonmammalian collagens have attracted significant attention owing to their potential for use as a source of cell scaffolds for tissue engineering. Since the morphology of collagen fibrils controls cell proliferation and differentiation, its regulation is essential for fabricating scaffolds with desirable characteristics. In this study, we evaluated the effects of the phosphate ion (Pi) concentration on the characteristics of fibrils formed from swim bladder type I collagen (SBC) and skin type I collagen (SC) from the Bester sturgeon. An increase in the Pi concentration decreased the fibril formation rate, promoted the formation of thick fibrils, and increased the thermal stability of the fibrils for both SBC and SC. However, the SBC and SC fibrils exhibited different fibril formation rates, degrees of fibrillogenesis, morphologies, and denaturation temperatures for the same reaction conditions. Finally, by regulating the Pi concentration, various types of SBC and SC fibrils could be coated on cell culture wells, and fibroblasts could be cultured on them. The results showed that thin fibrils enhance fibroblast extension and proliferation, whereas thick fibrils restrain fibroblast extension but orient them in the same direction. The results of this study suggest that SBC fibrils, which exhibit diverse morphologies, are suitable for use as a novel scaffold material, whose characteristics can be tailored readily by varying the Pi concentration. (C) 2020 Published by Elsevier B.V.
机译：由于它们用作组织工程的细胞支架来源，非含有胶原蛋白引起了显着的关注。由于胶原型原纤维的形态控制细胞增殖和分化，其调节对于制造具有所需特性的支架是必不可少的。在这项研究中，我们评估了磷酸离子（PI）浓度对由BESER Sturgeon的游泳膀胱型I胶原（SBC）和皮肤型I胶原（SC）形成的原纤维的特征的影响。 PI浓度的增加降低了原纤维形成速率，促进了厚纤维的形成，并增加了SBC和SC的原纤维的热稳定性。然而，SBC和SC原纤维具有不同的原纤维形成速率，原纤维发生程度，形态，以及相同的反应条件的变性温度。最后，通过调节PI浓度，可以在细胞培养孔上涂覆各种类型的SBC和SC原纤维，并且可以在它们上培养成纤维细胞。结果表明，薄的原纤维增强成纤维细胞延伸和增殖，而厚纤维抑制成纤维细胞延伸，但在相同方向上定向它们。本研究的结果表明，表现出不同形态的SBC原纤维是适合用作新的支架材料，其特性可以通过改变PI浓度来容易地定制。 （c）2020由elsevier b.v发布。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号