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首页> 外文期刊>International Journal of Biological Macromolecules: Structure, Function and Interactions >Structural characterization and antioxidant effect of green alga Enteromorpha prolifera polysaccharide in Caenorhabditis elegans via modulation of microRNAs
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Structural characterization and antioxidant effect of green alga Enteromorpha prolifera polysaccharide in Caenorhabditis elegans via modulation of microRNAs

机译：微RORNA调制CAENORHABDIOLANS绿藻肠道肌肉多糖的结构表征及抗氧化效果

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摘要

A water-soluble heteropolysaccharide was isolated and purified from Enteromorpha prolifera by DEAE-52 and Bio-Gel P-2 column chromatography. Fourier transform infrared spectroscopy (FTIR), high performance liquid chromatography (HPLC), multi-angle laser light scattering (MALLS), and nuclear magnetic resonance (NMR) spectroscopy were used to characterize the structure of E. prolifera polysaccharide degradation (EPP-1). Its anti-oxidative activity was determined in Caenorhabditis elegans via modulation of microRNAs. The average molecular weight of EPP-1 was 4.28 kDa. It contained six types of linkage units as -> 2)-beta-D-GlcpA-(1 ->, -> 3,6)-beta-D-Manp (1 ->, -> 4)-alpha-D-Glcp (1 ->, -> 6)-beta-D-Galp (1 ->, beta-L-Rhap-(->, and -> 4)-beta-D-GalpA-( 1 ->. The mean lifespan, ultraviolet-induced oxidative stress, and thermotoler- ance in C elegans were improved after treatment of EPP-1. Moreover, EPP-1 significantly increased the total superoxide dismutase levels and decreased the malondialdehyde levels in C. elegans. Intracellular reactive oxygen species accumulation and DNA damage were ameliorated by up-regulation of SKN-1 and DAF-16 expression through miR-48 and miR-51 miR-186 down-regulation. In vivo studies demonstrated that EPP-1 might be applied in functional foods as the antioxidative and anti-ageing ingredient. (C) 2019 Elsevier B.V. All rights reserved.
机译：水溶性杂多糖分离并通过DEAE-52和生物凝胶P-2柱色谱法从浒苔纯化。傅里叶变换红外光谱（FTIR），高效液相色谱法（HPLC），多角度激光光散射（MALLS），和核磁共振（NMR）光谱法来表征浒苔多糖降解（EPP-1的结构）。其抗氧化活性在秀丽隐杆线虫经由微RNA的调制来确定。 EPP-1的平均分子量为4.28千道尔顿。它包含了六类联动单位 - > 2）-β-d-GlcpA-（1 - >， - > 3,6）-β-d-manP启动（1 - >， - > 4）-α-D- GLCP（1 - >， - > 6）-β-d-高浦（1 - >，β-L-Rhap - （ - >和 - > 4）-β-d-GalpA-（1 - >平均寿命，紫外线诱导的氧化应激，并用C线虫thermotoler- ANCE被治疗EPP-1的后好转。此外，EPP-1显著增加总超氧化物歧化酶水平和线虫降低丙二醛的水平。细胞内活性氧类物质积累和DNA损伤是由SKN-1的上调和DAF-16表达通过的miR-48和miR-51的miR-186的下调改善。体内研究证明了EPP-1可能在功能性食品为被施加抗氧化和抗衰老成分。版权所有（C）2019爱思唯尔所有权利。

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