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首页> 外文期刊>International Journal of Biological Macromolecules: Structure, Function and Interactions >Bi-enzymatic virus-like bionanoreactors for the transformation of endocrine disruptor compounds
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Bi-enzymatic virus-like bionanoreactors for the transformation of endocrine disruptor compounds

机译：双酶病毒样生物甘露反应器，用于转化内分泌破坏剂化合物

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Endocrine disruptor compounds (EDCs) are pollutants able to alter both hormone synthesis and their regulation in animals and humans, thus, EDCs represent a risk for public health and for the environment. Cytochrome P450 enzymes (CYPs) are involved in the detoxification of a wide range of compounds, and it has been established that these enzymes produce the initial biotransformation of many EDCs. In this work, a bionanoreactor based on the encapsulation of an enhanced peroxygenase CYP(BM3)21B3 inside the capsid of bacteriophage P22 virus-like particles (VLPs) was designed and characterized. VLPs were functionalized with glucose oxidase to generate in situ hydrogen peroxide necessary to activate the transformation of bisphenol A, nonylphenol, 17 beta-estradiol, tri-closan, and resorcinol. Catalytic parameters, as well as the chemical nature of reaction products are presented. The enzymatic nanoreactors showed specific activities varying from 0.175 to 0.456 min(-1) in the transformation of these EDCs, which are equivalent to 22-77% of the activity obtained with free CYP. The capacity to transform structurally diverse compounds, easy production and glucose fueled catalytic activity make these enzymatic nanoreactors an interesting platform for enzyme delivery in the biomedical field. (C) 2020 Elsevier B.V. All rights reserved.
机译：内分泌破坏剂化合物（EDC）是能够改变激素合成及其在动物和人体中的调节的污染物，因此，EDC表示公共卫生和环境的风险。细胞色素P450酶（CYPS）参与了各种化合物的解毒，并且已经确定这些酶产生许多EDC的初始生物转化。在这项工作中，设计并表征了基于在噬菌体P22病毒样颗粒（VLP）的囊体内的增强的过氧酶CYP（BM3）21b3的封装的Bionanoreaccor。 VLP用葡萄糖氧化酶官能化，以产生原位氢过氧化氢，以激活双酚A，壬基酚，17β-雌二醇，三封和间苯二酚的转化。催化参数以及反应产物的化学性质。酶促纳米反应器显示在这些EDC的转化中不同于0.175至0.456 min（-1）的特异性活性，这相当于使用免费CYP获得的活性的22-77％。改变结构各种化合物的能力，易于生产和葡萄糖燃料催化活性使得这些酶纳米反应器成为生物医学领域中的酶递送的有趣平台。 （c）2020 Elsevier B.v.保留所有权利。

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