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首页> 外文期刊>International Journal of Biological Macromolecules: Structure, Function and Interactions >Silk-sericin degummed wastewater solution-derived and nitrogen enriched porous carbon nanosheets for robust biological imaging of stem cells
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Silk-sericin degummed wastewater solution-derived and nitrogen enriched porous carbon nanosheets for robust biological imaging of stem cells

机译：丝胺脱胶废水溶液 - 衍生的和氮富集多孔碳纳米液，用于干细胞的鲁棒生物成像

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摘要

Appreciated raw materials like silk-sericin can be recovered from silk-textile industrial waste for the production of novel functional nanomaterials. In this study, highly fluorescent sericin based carbon nanosheets (SCN) were produced from industrial wastewater containing silk-sericin as a precursor, and was applied as bio-imaging application for oral fat stem cells. A simple one-pot, hydrothermal carbonization method was used to produce SCN at a 180 degrees C. The obtained hydrothermal carbons exhibited strong fluorescence properties due to the presence of strong polar groups, such as carboxyl, amino and amide groups in the surface. Heteroatom functionalization of the SCN leads to the property of fluorescence due to enriched nitrogen and was confirmed by X-ray photoelectron and Fourier transform infrared spectroscopy. The plate-like morphology of SCN about 35 nm in size was evaluated by transmission electron microscopy. The carbon 13 nuclear magnetic resonance results revealed that nano-sized fluorescent SCN formed during carbonization and functionalization occurred through dehydration of the sericin protein. Moreover, the prepared SCNs demonstrated low toxicity and their suitability for bio-imaging applications was demonstrated to the oral fat stem cells. Overall, sericin degumming wastewater from the silk textile industry can be utilized for the production of SCNs for stem cells bio-imaging applications. (C) 2017 Elsevier B.V. All rights reserved.
机译：赞赏的原料可以从丝绸纺织工业废物中恢复，用于生产新型功能纳米材料。在该研究中，从含有丝丝蛋白的工业废水作为前体的工业废水制备了高度荧光胺基碳纳米片（SCN），并被用作口腔脂肪干细胞的生物显像施用。使用简单的单罐水热碳化法在180℃下产生SCN。所获得的水热碳由于存在强极性基团，例如羧基，氨基和酰胺基团而表现出强烈的荧光性能。 SCN的杂原子官能化导致由于富含氮的荧光性，并通过X射线光电子和傅立叶变换红外光谱证实。通过透射电子显微镜评估SCN的板状形态约35nm。碳13核磁共振结果表明，在碳化和官能化期间形成的纳米尺寸荧光SCN通过胺蛋白的脱水发生。此外，制备的SCNS表现出低毒性，并且对生物成像应用的适合性被证明于口腔脂肪干细胞。总体而言，丝绸纺织工业的胺蛋白脱胶废水可用于生产SCNS用于干细胞生物成像应用。 （c）2017年Elsevier B.V.保留所有权利。

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