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首页> 外文期刊>International Journal of Biological Macromolecules: Structure, Function and Interactions >Dissolution and regeneration of wool keratin in the deep eutectic solvent of choline chloride-urea
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Dissolution and regeneration of wool keratin in the deep eutectic solvent of choline chloride-urea

机译：胆碱氯化胆脲羊毛角蛋白羊毛角蛋白的溶解和再生

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摘要

Wool keratin is a kind of renewable protein which has great potential in environment-friendly materials. In this paper, the dissolution of wool fiber and the regeneration of wool keratin in the deep eutectic solvent (DES) of choline chloride-urea was detailed studied. The results showed that 35.1 mg wool completely dissolved in 1 g DES at 130 degrees C in 5 h. The polarizing microscope, scanning electron microscope (SEM) images along with the amino acid analysis suggested that the DES mainly dissolved the wool cortex layer. Compared with the raw wool, the regenerated wool keratin contained much more fragments of low molecular weight polypeptide chains from SDS-PAGE results. And the percentage of alpha-helix dramatically decreased, while the content of beta-sheet and disordered structure increased. All the results indicated that the DES can be used as a kind of green solvent for the regeneration of wool keratin without losing the long peptide chains. The method also offered a possibility of large-scale for wasted wool reuse. (C) 2018 Elsevier B.V. All rights reserved.
机译：羊毛角蛋白是一种可再生蛋白质，具有巨大的环保材料。本文研究了羊毛纤维的溶解和羊毛角蛋白在胆碱 - 氯化胆碱的深层共晶溶剂（DES）中的溶解。结果表明，35.1mg羊毛在5小时内将35.1mg羊毛完全溶解在1g des中。偏振显微镜，扫描电子显微镜（SEM）图像以及氨基酸分析表明DES主要溶解羊毛皮质层。与生羊毛相比，再生羊毛角蛋白来自SDS-PAGE结果的低分子量多肽链的碎片。并且α-螺旋的百分比显着降低，而β-薄片和无序结构的含量增加。所有结果表明，DES，可以使用作为一种用于羊毛角的再生绿色溶剂的不失长肽链。该方法还提供了大规模的羊毛再利用的可能性。 （c）2018年elestvier b.v.保留所有权利。

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