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首页> 外文期刊>International Journal of Biological Macromolecules: Structure, Function and Interactions >Cobalt oxide nanoparticles mediate tau denaturation and cytotoxicity against PC-12 cell line
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Cobalt oxide nanoparticles mediate tau denaturation and cytotoxicity against PC-12 cell line

机译：氧化钴纳米粒子介导Tau变性和细胞毒性对PC-12细胞系

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摘要

It has not been well explored how NPs may induce some adverse effects on the biological systems. In this research, the interaction of cobalt oxide NPs (Co3O4 NPs) with tau protein and PC-12 cell line, as nervous system models, was investigated with several approaches including fluorescence spectroscopy, CD spectroscopy, docking study, MTT, LDH, AO/EB dual staining, and flow cytometry assays. Fluorescence investigation displayed that Co3O4 NPs spontaneously mediate the formation of a static complex with tau protein through hydrogen bonds and van der Waals forces. Docking study also revealed that Ser and Gln residues play important roles in the formation of hydrogen bonds between tau and Co3O4 NPs. Far UV-CD measurement determined that Co3O4 NPs changed the unfolded structure of tau protein toward a more folded conformation. Moreover, Co3O4 NPs demonstrated to stimulate the reduction of PC-12 cell viability through membrane leakage, fragmentation of DNA, apoptosis, and necrosis. In conclusion, Co3O4 NPs may trigger marked alterations on the tertiary and secondary structure of tau protein. Also, the dose of Co3O4 NPs is the crucial factor which induces their adverse effects on the cells. Because, all side effects of NPs are not well explored, additional detailed experiments are more needed. (C) 2018 Elsevier B.V. All rights reserved.
机译：探索NPS可能诱导对生物系统的一些不利影响。在本研究中，用荧光光谱，CD光谱，对接研究，MTT，LDH，AO /的几种方法研究了钴氧化物NPS（CO3O4 NPS）与TAU蛋白和PC-12细胞系的相互作用，作为神经系统模型。 EB双染色和流式细胞术测定。荧光调查显示CO3O4 NPS通过氢键和范德瓦尔斯力自发地介导与TAU蛋白的静态复合物的形成。对接研究还揭示了Ser和Gln残留物在Tau和Co3O4 NPS之间形成氢键的形成。远程UV-CD测量确定CO3O4 NPS改变了TAU蛋白的展开结构朝向更折叠的构象。此外，CO3O4 NPS通过膜泄漏，DNA的破碎，细胞凋亡和坏死，刺激CO 3O4 NPS刺激PC-12细胞活力。总之，CO3O4 NPS可能触发TAU蛋白的第三级和二级结构的显着改变。此外，CO3O4 NP的剂量是诱导它们对细胞不利影响的关键因素。因为，NPS的所有副作用都不熟悉，更需要额外的详细实验。 （c）2018年elestvier b.v.保留所有权利。

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