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首页> 外文期刊>International Journal of Biological Macromolecules: Structure, Function and Interactions >Enhanced B-phycoerythrin production by the red microalga Porphyridium marinum: A powerful agent in industrial applications
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Enhanced B-phycoerythrin production by the red microalga Porphyridium marinum: A powerful agent in industrial applications

机译:增强了红色微藻卟啉摩利诺姆的B-植物治疗生产:工业应用中强大的药剂

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摘要

The production of B-phycoerythrin (B-PE) from the red microalga Porphyridium marinum was optimized before to purify it and subsequently study its antioxidant activities. NaNO3, K2HPO4 and metal traces concentrations of the culture medium, and luminosity parameters were chosen, according to the Plackett-Burman design, as the most influent factors on the B-PE production by P. marinum. The optimization of these factors according to the Box-Behnken plan gave a maximum of B-PE production equal to 40 mg/g dry weight under the following conditions: NaNO3 = 3.4 g/L; K2HPO4 = 0 g/L; light intensity = 70 mu mol photons/m(2)/s and metal solution = 1.5 mL/L. The B-PE also showed an interesting capacity to chelate Fe3+ (IC50 = 13.91 +/- 021 mu g/mL) and a significant reducing power (OD700nm = 0.485 +/- 0.011 at 100 mu g/mL). The present study reports the antioxidant potential of purified B-PE from P. marinum that could be potentially used as a source of bioactive protein for a wide range of cosmetic and pharmaceutical applications. (C) 2018 Elsevier B.V. All rights reserved.
机译:在纯化它之前优化了来自红色微藻卟啉Marinum的B- phycoerythrin(B-PE)的生产,并随后研究其抗氧化活性。根据Plackett-Burman设计,选择培养基,K2HPO4和金属痕量培养基和发光度参数的浓度,是P. Marinum的B-PE生产中最受影响的因素。根据盒-Behnken计划的这些因素的优化,在以下条件下,最多具有等于40mg / g干重等于40mg / g的干重;纳米3 = 3.4g / L; K2HPO4 = 0 g / l;光强度=70μmol光子/ m(2)/ s和金属溶液= 1.5ml / L. B-PE还显示出螯合Fe3 +(IC50 = 13.91 +/-021μg/ ml)的有趣能力,并且显着的还原功率(OD700nm = 0.485 +/- 0.011,在100μg/ ml)。本研究报告了来自P. Marinum的纯化B-PE的抗氧化潜力,其可能被潜在地用作各种化妆品和药物应用的生物活性蛋白质来源。 (c)2018年elestvier b.v.保留所有权利。

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