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首页> 外文期刊>International Journal of Biological Macromolecules: Structure, Function and Interactions >Influence of photoinitiator concentration and irradiation time on the crosslinking performance of visible-light activated pullulan-HEMA hydrogels
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Influence of photoinitiator concentration and irradiation time on the crosslinking performance of visible-light activated pullulan-HEMA hydrogels

机译：光引发剂浓度和辐射时间对可见光活性鳞叶血清水凝胶交联性能的影响

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摘要

In-situ forming hydrogels were prepared from pullulan-HEMA copolymer using three-component visible-light system composed of camphorquinone carboxylic acid-folic acid-iodonium salt. The relevance of double bond conversion and crosslinking density of hydrogels with the photoinitiator concentration and irradiation time were estimated by FT-IR analysis and swelling calculation using Flory-Rehner theory, respectively. The results revealed that the crosslinking density and degree of conversion of hydrogels were improved by photoinitiator concentration increasing until certain extend, then they decreased due to a primary radicals termination reaction occurred. The shortest irradiation time of 10 s was essential to obtain acceptable hydrogels for further characterizations. For the probability use of hydrogels as scaffold was investigated in vitro by measuring of the indirect cytotoxicity assay by MTT-assay using human bone Sarcoma cell as a reference cell lines. The majority of seeded SW1353 cells maintained a live with an accepted viability of similar to 85-92% over a four days culture period with irradiation of hydrogel 10 s, while cell viability has improved to similar to 95-98% with prolonging the irradiation time of hydrogel to 60 s. The current photoinitiating system is a proper system for in-situ crosslinking the activated-light biomaterials for bone regeneration, dental, or tissue engineering applications. (C) 2018 Elsevier B.V. All rights reserved.
机译：使用由樟脑醌羧酸 - 叶酸 - 碘鎓盐组成的三组分可见光系统由淀粉 - 肟共聚物制备原位形成水凝胶。通过FT-IR分析和溶胀计算，估计使用血液恢复理论估算了具有光引发剂浓度和辐射时间的水凝胶的双键转化和交联密度的相关性。结果表明，通过光引发剂浓度的增加，将水凝胶的交联密度和转化程度提高，直至某些延伸，然后它们由于初始自由基终止反应而降低。 10 s的最短照射时间对于获得可接受的水凝胶是必不可少的，以进行进一步表征。通过使用人骨Sarcoma电池作为参考细胞系测量MTT测定，在体外研究了水凝胶作为支架的概率使用。大多数种子SW1353细胞维持活力，在4天培养期与水凝胶10S的照射中相似的可接受的活力，而细胞活力会改善至95-98％，延长照射时间水凝胶到60秒。目前的光引发系统是用于原位交联的适当系统，用于骨再生，牙科或组织工程应用的活性光生物材料。 （c）2018年elestvier b.v.保留所有权利。

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