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首页> 外文期刊>International Journal of Biological Macromolecules: Structure, Function and Interactions >Bio-catalytic performance and dye-based industrial pollutants degradation potential of agarose-immobilized MnP using a Packed Bed Reactor System
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Bio-catalytic performance and dye-based industrial pollutants degradation potential of agarose-immobilized MnP using a Packed Bed Reactor System

机译：使用填充床反应器系统，生物催化性能和染料的工业污染物降解琼脂糖固定的MNP的潜力

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In this study, the matrix-entrapment technique was adopted to immobilize a novel manganese peroxidase (MnP). Agarose beads developed from 3.0% agarose concentration furnished the preeminent immobilization yield (92.76%). The immobilized MnP exhibited better resistance to changes in the pH and temperature as compared to the free counterpart, with optimal conditions being pH 6.0 and 45 degrees C. Thermal and storage stability characteristics were significantly improved after immobilization, and the immobilized-MnP displayed higher tolerance against different temperatures than free MnP state. After 72h, the insolubilized MnP retained its activity up to 41.2 +/- 1.7% and 33.6 +/- 1.4% at 55 degrees C and 60 degrees C, respectively, and 34.3 +/- 1.9% and 22.0 +/- 1.1% activities at 65 degrees C and 70 degrees C, respectively, after 48 h of the incubation period. A considerable reusability profile was recorded with ten consecutive cycles. Moreover, to explore the industrial applicability, the agarose-immobilized-MnP was tested for bioremediation of textile industry effluent purposes. After six consecutive cycles, the tested effluents were decolorized to different extents (with a maximum of 98.4% decolorization). In conclusion, the remarkable bioremediation potential along with catalytic, thermo-stability, reusability, as well as storage stability features of the agarose-immobilized-MnP reflect its prospects as a biocatalyst for bioremediation and other industrial applications. (C) 2017 Elsevier B.V. All rights reserved.
机译：在该研究中，采用基质缩醛技术固定新型锰过氧化物酶（MNP）。从3.0％琼脂糖浓度开发的琼脂糖珠子提供了卓越的固定产率（92.76％）。与游离对应物相比，固定的MNP对pH和温度的变化表现出更好的抵抗力，最佳条件是pH 6.0和45℃。固定后，热和储存稳定性特性显着改善，并且固定的-MNP显示出更高的耐受性与免费的MNP状态不同的温度。 72h后，不溶性的MNP在55摄氏度和60摄氏度下保留其活性至41.2 +/- 1.7％和33.6 +/- 1.4％，以及34.3 +/- 1.9％和22.0 +/- 1.1％的活动在48小时后，在65摄氏度和70摄氏度下，在孵育期48小时后。有一个相当的可重用配置文件录制了十个连续周期。此外，为了探讨工业实用性，对纺织工业出水目的的生物修复进行了琼脂糖 - 固定的MNP。连续六个循环后，将测试的流出物脱色到不同的范围内（最多98.4％的脱色）。总之，显着的生物修复潜力以及催化，热稳定性，可重用性以及琼脂糖 - 固定的-MNP的储存稳定性特征反映了其作为生物化和其他工业应用的生物催化剂的前景。 （c）2017年Elsevier B.V.保留所有权利。

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