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首页> 外文期刊>International Journal of Biological Macromolecules: Structure, Function and Interactions >Galactomannan from Schizolobium amazonicum seed and its sulfated derivatives impair metabolism in HepG2 cells
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Galactomannan from Schizolobium amazonicum seed and its sulfated derivatives impair metabolism in HepG2 cells

机译：来自Schizolobium amazonicum种子的半乳甘露乳甘露乳甘露乳甘露乳甘露甘露糖醛植物在Hepg2细胞中损害代谢

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摘要

This study evaluated the effects of native galactomannan from Schizolobium amazonicum seeds and its sulfated forms on certain metabolic parameters of HepG2 cells. Aqueous extraction from S. amazonicum seeds furnished galactomannan with 3.2:1 Man:Gal ratio (SAGM) and molar mass of 4.34 x 10(5) g/mol. The SAGM fraction was subjected to sulfation using chlorosulfonic acid to obtain SAGMS1 and SAGMS2 with DS of 0.4 and 0.6, respectively. Cytotoxicity of SAGM, SAGMS1, and SAGMS2 was evaluated in human hepatocellular carcinoma cells (HepG2). After 72 h, SAGM decreased the viability of HepG2 cells by 50% at 250 mu g/mL, while SAGMS1 reduced it by 30% at the same concentration. SAGM, SAGMS1, and SAGMS2 promoted a reduction in oxygen consumption and an increase in lactate production in non-permeabilized HepG2 cells after 72 h of treatment. These results suggest that SAGM, SAGMS1, and SAGMS2 could be recognized by HepG2 cells and might trigger alterations that impair its survival. These effects could be implicated in the modification of the oxidative phosphorylation process in HepG2 cells and activation of the glycolytic pathway. (C) 2017 Elsevier B.V. All rights reserved.
机译：该研究评估了天然半乳甘露甘露甘露甘露甘露甘露聚糖的影响及其硫酸化形式对HepG2细胞的某些代谢参数。 S. Amazonicum种子的水性提取家具加乳甘露甘露甘露甘油，3.2：1人：GAL比（SAGM）和摩尔质量为4.34×10（5）克/摩尔。使用氯磺酸进行SAGM级分，以分别获得DS为0.4和0.6的SAGMS1和SAGMS2。在人肝细胞癌细胞（Hepg2）中评估了Sagm，Sagms1和Sagms2的细胞毒性。在72小时后，SAGM在250μg/ ml下将HepG2细胞的活力降低50％，而SAGMS1以相同的浓度将其减少30％。 SAGM，SAGMS1和SAGMS2促进氧气消耗的降低，并在72小时后在非渗透HepG2细胞中增加乳酸盐产生。这些结果表明，HepG2细胞可以识别SAGM，SAGMS1和SAGMS2，可能会触发损害其存活的变化。这些效果可以涉及在HepG2细胞中氧化磷酸化方法的改性和糖粘糊性途径的激活。 （c）2017年Elsevier B.V.保留所有权利。

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